Demonstration of Physical Interactions between Consecutive Enzymes of the Citric Acid Cycle and of the Aspartate‐Malate Shuttle
10.1111/j.1432-1033.1981.tb06369.x
Crossref journal-article
Wiley
European Journal of Biochemistry (311)
Abstract

By means of covalently immobilized fumarase and mitochondrial or cytoplasmic malate dehydrogenase we were able to detect physical interactions between different enzymes of the citric acid cycle (fumarase with malate dehydrogenase, malate dehydrogenase with citrate synthase and fumarase with citrate synthase) and between the enzymes of both mitochondria) and cytoplasmic halves of the aspartate‐malate shuttle (aspartate aminotransierase and malate dehydrogenase). The interactions between fumarase and malate dehydrogenase were also investigated by immobilizing one enzyme indirectly through antibodies bound to Sepharose‐protein A. Our results are consistent with a model in which maximally four molecules of malate dehydrogenase are bound to one fumarase molecule. This complex is able to bind either citrate synthase or aspartate aminotransferase. We propose that these enzymes bind alternatively, in order to allow the cell to perform citric acid cycle or shuttle reactions, according to its needs. The physiological meaning and implications on the regulation of metabolism of the existence of a large citric acid cycle/malate‐aspartate shuttle multienzyme complex are discussed.

Bibliography

BEECKMANS, S., & KANAREK, L. (1981). Demonstration of Physical Interactions between Consecutive Enzymes of the Citric Acid Cycle and of the Aspartate‐Malate Shuttle. European Journal of Biochemistry, 117(3), 527–535. Portico.

Authors 2
  1. Sonia BEECKMANS (first)
  2. Louis KANAREK (additional)
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Dates
Type When
Created 15 years, 1 month ago (July 21, 2010, 1:22 p.m.)
Deposited 1 year, 11 months ago (Oct. 1, 2023, 10:46 p.m.)
Indexed 2 months, 1 week ago (June 23, 2025, 9:45 a.m.)
Issued 44 years, 2 months ago (July 1, 1981)
Published 44 years, 2 months ago (July 1, 1981)
Published Online 20 years, 5 months ago (March 3, 2005)
Published Print 44 years, 2 months ago (July 1, 1981)
Funders 0

None

@article{BEECKMANS_1981, title={Demonstration of Physical Interactions between Consecutive Enzymes of the Citric Acid Cycle and of the Aspartate‐Malate Shuttle: A Study Involving Fumarase, Malate Dehydrogenase, Citrate Synthase and Aspartate Aminotransferase}, volume={117}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb06369.x}, DOI={10.1111/j.1432-1033.1981.tb06369.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BEECKMANS, Sonia and KANAREK, Louis}, year={1981}, month=jul, pages={527–535} }