DOI: 10.1111/j.1432-1033.1981.tb06310.x. An α‐Amanitin‐Resistant DNA‐Dependent RNA Polymerise II from the Fungus <i>Aspergillus nidulans</i>

An α‐Amanitin‐Resistant DNA‐Dependent RNA Polymerise II from the Fungus Aspergillus nidulans

10.1111/j.1432-1033.1981.tb06310.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

An α‐amanitin‐resistant DNA‐dependent RNA polymerise II has been purified from the lower eukaryote Aspergillus nidulans to apparent homogeneity by extraction of the enzyme at low salt concentration, polymin P (polyethylene imine) fractionation, binding to ion‐exchangers and density gradient centrifugation. By this procedure 0.4 mg of RNA polymerise II can be purified over 6000‐fold from 500 g (wet weight) of starting material with a yield of 25% and a specific activity of 550 units/mg.The subunit composition has been resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate and by two‐dimensional gel electrophoresis using a non‐denaturing gel in the first dimension and a dodecylsulphate slab gel in the second dimension. The putative subunits have molecular weights of 170000, 150000, 33000, 27000, 24000, 19000, 18000 and 16000. Only one form of RNA polymerise II could be resolved by electrophoresis. The chromatographic and catalytic properties and the subunit composition of the purified RNA polymerise II are clearly different from RNA polymerise I from A. nidulans but throughout comparable with other class II enzymes. It differs from all other class II enzymes by its insensitivity towards the toxin α‐amanitin, even at concentrations up to 400 μg/ml, and appears to be unable to bind O‐[14C]methyl‐γ‐amanitin at a concentration of 10 μg/ml of the toxin.We conclude that the purified RNA polymerise from A. nidulans is a real, but exceptional, type of the class II RNA polymerises.

Bibliography

STUNNENBERG, H. G., WENNEKES, L. M. J., SPIERINGS, T., & van den BROEK, H. W. J. (1981). An Î±âAmanitinâResistant DNAâDependent RNA Polymerise II from the Fungus Aspergillus nidulans. European Journal of Biochemistry, 117(1), 121â129. Portico.

Authors 4

Hendrik G. STUNNENBERG (first)
Lambertus M. J. WENNEKES (additional)
Titus SPIERINGS (additional)
Hendrikus W. J. van den BROEK (additional)

References 37 Referenced 15

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Dates

Type	When
Created	15 years, 1 month ago (July 21, 2010, 1 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 1:37 a.m.)
Indexed	1 day, 19 hours ago (Sept. 3, 2025, 6:38 a.m.)
Issued	44 years, 3 months ago (June 1, 1981)
Published	44 years, 3 months ago (June 1, 1981)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	44 years, 3 months ago (June 1, 1981)

Funders 0

None

BibTeX

@article{STUNNENBERG_1981, title={An α‐Amanitin‐Resistant DNA‐Dependent RNA Polymerise II from the Fungus Aspergillus nidulans}, volume={117}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb06310.x}, DOI={10.1111/j.1432-1033.1981.tb06310.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={STUNNENBERG, Hendrik G. and WENNEKES, Lambertus M. J. and SPIERINGS, Titus and van den BROEK, Hendrikus W. J.}, year={1981}, month=jun, pages={121–129} }

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