DOI: 10.1111/j.1432-1033.1981.tb06167.x. Fluorimetry Study of <i>N</i>‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin

Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin

10.1111/j.1432-1033.1981.tb06167.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A fluorescent reagent, N‐(1‐pyrenyl)iodoacetamide, was conjugated to rabbit skeletal muscle actin at the site of the most reactive sulfhydryl group, and fluorescence characteristics (excitation and emission spectra, quantum yields, lifetimes) of the conjugate were investigated. Associated with polymerization of labelled G‐actin, the fluorescence intensity at 407 nm, after excitation at 365 nm, was enhanced by a factor of about 25. It was reduced to about 25% on the binding of heavy meromyosin (or subfragment 1). The results suggest that binding of heavy meromyosin to the protomer of F‐actin alters the local structure of the protomer towards a G‐actin‐like one.

Bibliography

KOUYAMA, T., & MIHASHI, K. (1981). Fluorimetry Study of Nâ(1âPyrenyl)iodoacetamideâLabelled FâActin. European Journal of Biochemistry, 114(1), 33â38. Portico.

Authors 2

Tsutomu KOUYAMA (first)
Koshin MIHASHI (additional)

References 13 Referenced 707

{'key': 'e_1_2_2_2_2', 'first-page': '261', 'volume-title': 'Subunits in Biological Systems', 'author': 'Oosawa F.', 'year': '1971'} / Subunits in Biological Systems by Oosawa F. (1971)
10.1016/S0021-9258(18)62016-2
10.1093/oxfordjournals.jbchem.a132341 / J. Biochem. (Tokyo) by Mihashi K. (1979)
10.1073/pnas.70.9.2687
10.1016/S0022-2836(77)80119-8
10.1038/257054a0
10.1111/j.1432-1033.1980.tb04499.x
10.1016/0003-2697(67)90174-1
10.1016/S0021-9258(19)41137-X
10.1016/S0021-9258(17)34749-X / J. Biol. Chem. by Betcher‐Lange S. L. (1978)
10.1016/S0022-2836(62)80112-0
Kouyama T.(1980)Ph. D. Thesis Nagoya University.
10.1016/0022-2836(76)90181-9

Dates

Type	When
Created	15 years, 1 month ago (July 21, 2010, 10:28 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:02 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 5, 2025, 9:10 a.m.)
Issued	44 years, 6 months ago (Feb. 1, 1981)
Published	44 years, 6 months ago (Feb. 1, 1981)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years, 6 months ago (Feb. 1, 1981)

Funders 0

None

BibTeX

@article{KOUYAMA_1981, title={Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin: Local Structural Change of Actin Protomer both on Polymerization and on Binding of Heavy Meromyosin}, volume={114}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb06167.x}, DOI={10.1111/j.1432-1033.1981.tb06167.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KOUYAMA, Tsutomu and MIHASHI, Koshin}, year={1981}, month=feb, pages={33–38} }

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