DOI: 10.1111/j.1432-1033.1981.tb05685.x. Conformation of Chromatin Oligomers

10.1111/j.1432-1033.1981.tb05685.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Quasielastic laser light scattering measurements have been made on chromatin oligomers to obtain information on the transition in their electrooptical properties, previously observed for the hexaneric structures [Marion, C. and Roux, B. (1978) Nucleic Acids Res. 5, 4431‐4449]. Translational diffusion coefficients were determined for mononucleosomes to octanucleosomes containing histone H1 over a range of ionic strength. At hign ionic strength, oligomers show a linear dependence of the diffusion coefficient upon the logarithm of number of nucleosomes. At low ionic strength a change occurs between hexamer and heptamer. Our results agree well with the recent sedimentation data of Osipova et al. [Eur. J. Biochem. (1980) 113, 183 –188] and of butler and Thomas [J. Mol. Biol.(1980) 140, 505–529] showing a change in stability with hexamer. Various models for the arrangements of nucleosomes in the superstructure of chromatin are discussed. All calculations clearly indicate a conformational change with the hexanucleosome and the results suggest that, at low ionic strength, the chromatin adopts a loosely helical structure of 28‐nm diameter and 22‐nm pitch. These results are also consitent with a discontinuity every sixth necleosome, corresponding to a turn of the helix. This discontinuity may explain the recent electric dichroism data of Lee et al. [Biochemistry (1981) 20, 1438–1445]. The hexanucleosome structure which we have previously suggested, with the faces of nucleosomes arranged radially to the helical axis has been recently confirmed by Mc Ghee et al. [Cell (1980) 22,87–96].With an increase of ionic strength, the helix becomes more regular and compact with a slightly reduced outer diameter and a decreased pitch, the dimensions resembling those proposed for solenoid models.

Bibliography

MARION, C., BEZOT, P., HESSEâBEZOT, C., ROUX, B., & BERNENGO, J. (1981). Conformation of Chromatin Oligomers. European Journal of Biochemistry, 120(1), 169â176. Portico.

Authors 5

Christian MARION (first)
Pierre BEZOT (additional)
Christine HESSE‐BEZOT (additional)
Bernard ROUX (additional)
Jean‐Claude BERNENGO (additional)

References 56 Referenced 20

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 4:29 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 5:53 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 6:10 p.m.)
Issued	43 years, 10 months ago (Nov. 1, 1981)
Published	43 years, 10 months ago (Nov. 1, 1981)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	43 years, 10 months ago (Nov. 1, 1981)

Funders 0

None

BibTeX

@article{MARION_1981, title={Conformation of Chromatin Oligomers: A New Argument for a Change with the Hexanuclesome}, volume={120}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05685.x}, DOI={10.1111/j.1432-1033.1981.tb05685.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={MARION, Christian and BEZOT, Pierre and HESSE‐BEZOT, Christine and ROUX, Bernard and BERNENGO, Jean‐Claude}, year={1981}, month=nov, pages={169–176} }

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  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1981.tb05685.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1981,
        11
      ]
    ]
  }
}