DOI: 10.1111/j.1432-1033.1981.tb05588.x. Isoleucyl‐tRNA Synthetase from Baker's Yeast

Isoleucyl‐tRNA Synthetase from Baker's Yeast

10.1111/j.1432-1033.1981.tb05588.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The order of substrate addition to isoleucyl‐tRNA synthetase from baker's yeast has been investigated by steady‐state kinetics with inhibition by four different inhibiting ATP analogs acting competitively, uncompetitively and noncopetitively with respect to ATP, namely purineriboside (=nbularin), 3′‐deoxy‐adenosine (=cordycepin), 8‐amino‐adenosine and 8‐azido‐adenosine 5′‐triphosphates. The inhibition studies were done in the amino‐acylation and in the pyrophosphate exchange reaction, the aminoacylation eas investihated in the absence and presence of inorganic pyrophosphatase. Additionally bisubstrate kinetics and product inhibition studies were carried out.The inhibition patterns indicate a multisite system with a minimum number of two sites for each of the substrates. Yhe results of the pyrophosphate exchange studies are consistent with formation of E. Ile‐AMP‐ATP.Ile complexes by random addition of one ATP and one isoleucine molecule, followed by adenylate formation, subsequent release of pyrophosphate and random addition of a second molecule of ATP and iso‐leucine. For the aminoacylation in the absence of pyrophosphatase an ordered ter‐ter mechanism is postulated; in the presence of pyrophosphatase the mechanism is random bi‐uni uni‐bi ping‐pong. Both the pyrophosphate and the analogs of this compound such as imidodiphosphate or methylenediphosphonate can induce the enzyme to act in the ter‐ter mechanism.

Bibliography

FREIST, W., VON DER HAHR, F., & CRAMER, F. (1981). IsoleucylâtRNA Synthetase from Bakerâs Yeast. European Journal of Biochemistry, 119(1), 151â164. Portico.

Authors 3

Wolfgang FREIST (first)
Frsiedrich VON DER HAHR (additional)
Friedrich CRAMER (additional)

References 51 Referenced 12

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{'key': 'e_1_2_3_18_2', 'first-page': '82', 'volume': '12', 'author': 'Loftfield R. B.', 'year': '1972', 'journal-title': 'Progr. Nucleic Acid Res. Mol. Biol.'} / Progr. Nucleic Acid Res. Mol. Biol. by Loftfield R. B. (1972)
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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 4:19 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 10:42 p.m.)
Indexed	12 hours, 11 minutes ago (Sept. 4, 2025, 10:01 a.m.)
Issued	44 years ago (Sept. 1, 1981)
Published	44 years ago (Sept. 1, 1981)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	44 years ago (Sept. 1, 1981)

Funders 0

None

BibTeX

@article{FREIST_1981, title={Isoleucyl‐tRNA Synthetase from Baker’s Yeast: Action of ATP Analogs in Pyrophosphate Exchange and Aminoacylation, Two Pathways of the Aminoacylation Depending on Concentration of Pyrophosphate}, volume={119}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05588.x}, DOI={10.1111/j.1432-1033.1981.tb05588.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={FREIST, Wolfgang and VON DER HAHR, Frsiedrich and CRAMER, Friedrich}, year={1981}, month=sep, pages={151–164} }

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