DOI: 10.1111/j.1432-1033.1981.tb05499.x. Synthetic Substrates for Thyroid Oligosaccharide Transferase

Synthetic Substrates for Thyroid Oligosaccharide Transferase

10.1111/j.1432-1033.1981.tb05499.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The N‐glycosylation of proteins is initiated by transfer of preassembled oligosaccharides from lipid carriers to an asparagine residue in the sequence Asn‐Xaa‐Ser(Thr). Various synthetic peptides were previously shown to act as effective acceptors when supplied to microsomal membranes. The present work was undertaken to delineate further within such peptides the structural determinants required for the N‐glycosylation process. Several new peptides were synthesized to evaluate the effects of the chain length and of the modifications on the asparagine, Xaa or threonine residues: asparagine was replaced by glutamine, Xaa by proline or N‐methylated alanine, threonine by an homoserine residue. Furthermore, both side chains of asparagine and threonine were simultaneously replaced by thaw of glutamine and homoserine respectively to restore a putative hydrogen bonding between the carbonyl and hydroxyl groups. The assays were performed with a solubilized form of the oligosaccharide transferase and the acceptor capacities of the peptides expressed by the ratio V/Km of their apparent kinetic parameters V and Km. Results showed that N‐acetyl‐tripeptide amides are excellent substrates for the enzyme. All modifications in the‐Asn‐Xaa‐Thr‐region resulted in a loss of acceptor capacity. The importance of the conformational aspect is discussed.

Bibliography

RONIN, C., GRANIER, C., CASETI, C., BOUCHILLOUX, S., & Van RIETSCHOTEN, J. (1981). Synthetic Substrates for Thyroid Oligosaccharide Transferase. European Journal of Biochemistry, 118(1), 159â164. Portico.

Authors 5

Catherine RONIN (first)
Claude GRANIER (additional)
Christiane CASETI (additional)
Simone BOUCHILLOUX (additional)
Jurphaas Van RIETSCHOTEN (additional)

References 26 Referenced 38

{'key': 'e_1_2_3_2_2', 'first-page': '17', 'volume': '40', 'author': 'Marshall R. D.', 'year': '1974', 'journal-title': 'Biochem. Soc. Symp.'} / Biochem. Soc. Symp. by Marshall R. D. (1974)
10.1016/0304-4157(79)90006-6
10.1073/pnas.74.1.134
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10.1111/j.1432-1033.1979.tb19748.x / Eur. J. Biochem. by Bause E. (1980)
10.1002/jss.400080105
10.1016/0014-5793(78)81089-8
10.1016/S0021-9258(19)83579-2
10.1016/0014-5793(79)80559-1
{'key': 'e_1_2_3_12_2', 'first-page': '335', 'volume-title': 'Peptides', 'author': 'Rietschoten J.', 'year': '1979'} / Peptides by Rietschoten J. (1979)
10.1016/0014-5793(80)80623-5
10.1016/S0021-9258(17)34435-6
{'key': 'e_1_2_3_15_2', 'first-page': '1', 'volume-title': 'The Peptides, Analysis, Synthesis, Biology', 'author': 'Barany G.', 'year': '1980'} / The Peptides, Analysis, Synthesis, Biology by Barany G. (1980)
10.1016/0003-2697(70)90146-6
10.1021/jm00264a010
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{'key': 'e_1_2_3_20_2', 'first-page': '723', 'volume': '5', 'author': 'Mauguen Y.', 'year': '1976', 'journal-title': 'Cryst. Struct. Commun.'} / Cryst. Struct. Commun. by Mauguen Y. (1976)
10.1007/978-1-4684-1006-8_2
10.1111/j.1432-1033.1974.tb03615.x
10.1016/S0021-9258(19)70593-6
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10.1016/0006-291X(77)90962-7
10.1038/269775a0 / Nature (Lond.) by Rothman J. E. (1977)
10.1002/jss.400110103

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 4:15 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:20 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 4:58 p.m.)
Issued	44 years ago (Aug. 1, 1981)
Published	44 years ago (Aug. 1, 1981)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years ago (Aug. 1, 1981)

Funders 0

None

BibTeX

@article{RONIN_1981, title={Synthetic Substrates for Thyroid Oligosaccharide Transferase: Effects of Peptide Chain Length and Modifications in the‐Asn‐Xaa‐Thr‐Region}, volume={118}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05499.x}, DOI={10.1111/j.1432-1033.1981.tb05499.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={RONIN, Catherine and GRANIER, Claude and CASETI, Christiane and BOUCHILLOUX, Simone and Van RIETSCHOTEN, Jurphaas}, year={1981}, month=aug, pages={159–164} }

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