DOI: 10.1111/j.1432-1033.1981.tb05486.x. Biosynthesis of the Light‐Harvesting Chlorophyll <i>a/b</i> Protein

Biosynthesis of the Light‐Harvesting Chlorophyll a/b Protein

10.1111/j.1432-1033.1981.tb05486.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

When etiolated pea seedlings were exposed to continuous light for 24 h and then returned to darkness, 38% of the chlorophyll a, 74% of the chlorophyll b and 84% of the light‐harvesting chlorophyll a/b protein that had accumulated under illumination proved to be unstable in darkness. The unstable chlorophyll displayed a half‐life of abdüt 90 min. In contrast, the α and β subunits of the chloroplast coupling factor and the large and small subunits of ribulose 1,5‐bisphosphate carboxylase continued to accumulate in darkness, although at a slower rate than in plants maintained under light. Short‐term labelling in vivo with L‐[35S]methionine showed that leaves continued to synthesize the light‐harvesting protein and the small subunit of ribulose 1,5‐bisphosphate carboxylase for up to 48 h after transfer of plants from light to darkness. However, after long‐term labelling (16 h), the light‐harvesting chlorophyll a/b protein was found to be labelled to high specific activity only in illuminated leaves. I conclude that the light‐harvesting chlorophyll a/b protein is subject to turnover after transfer of plants from light to darkness. The site of breakdown appears to be the photosynthetic membrane. I suggest that turnover of the protein is part of the normal physiological mechanism for co‐ordinating the accumulation of the pigment and protein components of the light‐harvesting chlorophyll a/b complex.

Bibliography

BENNETT, J. (1981). Biosynthesis of the LightâHarvesting Chlorophyll a/b Protein. European Journal of Biochemistry, 118(1), 61â70. Portico.

Authors 1

John BENNETT (first)

References 39 Referenced 187

10.1111/j.1751-1097.1979.tb07840.x
10.1016/0968-0004(79)90297-4
10.1016/0005-2728(77)90137-2
10.1016/0003-9861(80)90424-5
10.1016/S0021-9258(17)30294-6
10.1016/B978-0-12-294350-8.50009-X
10.1146/annurev.pp.29.060178.002021
10.1073/pnas.77.9.5253
10.1016/0014-5793(72)80763-4
10.1111/j.1432-1033.1978.tb12273.x
Schmidt G. W. Cashmore A. R. Broadhurst M. K. Bartlett S.&Chua N. ‐H.(1980)Cold Spring Harbor Symp. Quant. Biol. in the press.
10.1038/285625a0
10.1111/j.1432-1033.1979.tb13101.x
10.1073/pnas.75.10.4749
10.1104/pp.65.4.641
10.1016/B978-0-12-220106-6.50010-3
{'key': 'e_1_2_3_18_2', 'first-page': '99', 'volume-title': 'International Symposiun on Chioroplast Development', 'author': 'Griffiths W. T.', 'year': '1978'} / International Symposiun on Chioroplast Development by Griffiths W. T. (1978)
{'key': 'e_1_2_3_19_2', 'first-page': '200', 'volume': '11', 'author': 'H. Machold O.', 'year': '1977', 'journal-title': 'Photosynthetica'} / Photosynthetica by H. Machold O. (1977)
10.1104/pp.55.4.763
10.1016/0003-9861(76)90484-7
10.1111/j.1399-3054.1969.tb07377.x
10.1073/pnas.73.12.4560
10.1104/pp.24.1.1
10.1111/j.1432-1033.1974.tb03599.x
10.1038/269344a0
10.1111/j.1432-1033.1979.tb13239.x
10.1038/227680a0
10.1016/S0021-9258(19)41496-8
10.1016/0003-9861(78)90031-0
10.1016/0014-5793(78)80760-1
{'key': 'e_1_2_3_32_2', 'volume': '42', 'author': 'Kupke D. W.', 'year': '1962', 'journal-title': 'Plant Physiol.'} / Plant Physiol. by Kupke D. W. (1962)
10.1104/pp.47.2.252
10.1016/0005-2787(74)90162-2
{'key': 'e_1_2_3_35_2', 'first-page': '641', 'volume-title': 'Internartonal Symposium on Chloroplast Development', 'author': 'Edelwan M.', 'year': '1978'} / Internartonal Symposium on Chloroplast Development by Edelwan M. (1978)
10.1104/pp.64.5.828
10.1104/pp.65.4.663
10.1104/pp.65.4.680
10.1104/pp.63.2.237
10.1007/BF00390180

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 4:15 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:18 a.m.)
Indexed	3 weeks, 4 days ago (Aug. 6, 2025, 8:01 a.m.)
Issued	44 years ago (Aug. 1, 1981)
Published	44 years ago (Aug. 1, 1981)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years ago (Aug. 1, 1981)

Funders 0

None

BibTeX

@article{BENNETT_1981, title={Biosynthesis of the Light‐Harvesting Chlorophyll a/b Protein: Polypeptide Turnover in Darkness}, volume={118}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05486.x}, DOI={10.1111/j.1432-1033.1981.tb05486.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BENNETT, John}, year={1981}, month=aug, pages={61–70} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T12:01:56Z",
    "timestamp": 1754481716341
  },
  "reference-count": 39,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 8615,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1981,
        8
      ]
    ]
  },
  "abstract": "<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>When etiolated pea seedlings were exposed to continuous light for 24 h and then returned to darkness, 38% of the chlorophyll <jats:italic>a</jats:italic>, 74% of the chlorophyll <jats:italic>b</jats:italic> and 84% of the light\u2010harvesting chlorophyll <jats:italic>a/b</jats:italic> protein that had accumulated under illumination proved to be unstable in darkness. The unstable chlorophyll displayed a half\u2010life of abd\u00fct 90 min. In contrast, the \u03b1 and \u03b2 subunits of the chloroplast coupling factor and the large and small subunits of ribulose 1,5\u2010bisphosphate carboxylase continued to accumulate in darkness, although at a slower rate than in plants maintained under light.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Short\u2010term labelling <jats:italic>in vivo</jats:italic> with L\u2010[<jats:sup>35</jats:sup>S]methionine showed that leaves continued to synthesize the light\u2010harvesting protein and the small subunit of ribulose 1,5\u2010bisphosphate carboxylase for up to 48 h after transfer of plants from light to darkness. However, after long\u2010term labelling (16 h), the light\u2010harvesting chlorophyll <jats:italic>a/b</jats:italic> protein was found to be labelled to high specific activity only in illuminated leaves.</jats:p></jats:list-item>\n<jats:list-item><jats:p>I conclude that the light\u2010harvesting chlorophyll <jats:italic>a/b</jats:italic> protein is subject to turnover after transfer of plants from light to darkness. The site of breakdown appears to be the photosynthetic membrane. I suggest that turnover of the protein is part of the normal physiological mechanism for co\u2010ordinating the accumulation of the pigment and protein components of the light\u2010harvesting chlorophyll <jats:italic>a/b</jats:italic> complex.</jats:p></jats:list-item>\n</jats:list>\n</jats:p>",
  "DOI": "10.1111/j.1432-1033.1981.tb05486.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T21:15:41Z",
    "timestamp": 1109884541000
  },
  "page": "61-70",
  "source": "Crossref",
  "is-referenced-by-count": 187,
  "title": "Biosynthesis of the Light\u2010Harvesting Chlorophyll <i>a/b</i> Protein",
  "prefix": "10.1111",
  "volume": "118",
  "author": [
    {
      "given": "John",
      "family": "BENNETT",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1751-1097.1979.tb07840.x"
    },
    {
      "key": "e_1_2_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(79)90297-4"
    },
    {
      "key": "e_1_2_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(77)90137-2"
    },
    {
      "key": "e_1_2_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(80)90424-5"
    },
    {
      "key": "e_1_2_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)30294-6"
    },
    {
      "key": "e_1_2_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-294350-8.50009-X"
    },
    {
      "key": "e_1_2_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.pp.29.060178.002021"
    },
    {
      "key": "e_1_2_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.77.9.5253"
    },
    {
      "key": "e_1_2_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(72)80763-4"
    },
    {
      "key": "e_1_2_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1978.tb12273.x"
    },
    {
      "key": "e_1_2_3_12_2",
      "unstructured": "Schmidt G. W. Cashmore A. R. Broadhurst M. K. Bartlett S.&Chua N. \u2010H.(1980)Cold Spring Harbor Symp. Quant. Biol. in the press."
    },
    {
      "key": "e_1_2_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/285625a0"
    },
    {
      "key": "e_1_2_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1979.tb13101.x"
    },
    {
      "key": "e_1_2_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.75.10.4749"
    },
    {
      "key": "e_1_2_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.65.4.641"
    },
    {
      "key": "e_1_2_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-220106-6.50010-3"
    },
    {
      "key": "e_1_2_3_18_2",
      "first-page": "99",
      "volume-title": "International Symposiun on Chioroplast Development",
      "author": "Griffiths W. T.",
      "year": "1978"
    },
    {
      "key": "e_1_2_3_19_2",
      "first-page": "200",
      "volume": "11",
      "author": "H. Machold O.",
      "year": "1977",
      "journal-title": "Photosynthetica"
    },
    {
      "key": "e_1_2_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.55.4.763"
    },
    {
      "key": "e_1_2_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(76)90484-7"
    },
    {
      "key": "e_1_2_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-3054.1969.tb07377.x"
    },
    {
      "key": "e_1_2_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.73.12.4560"
    },
    {
      "key": "e_1_2_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.24.1.1"
    },
    {
      "key": "e_1_2_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1974.tb03599.x"
    },
    {
      "key": "e_1_2_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/269344a0"
    },
    {
      "key": "e_1_2_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1979.tb13239.x"
    },
    {
      "key": "e_1_2_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/227680a0"
    },
    {
      "key": "e_1_2_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)41496-8"
    },
    {
      "key": "e_1_2_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(78)90031-0"
    },
    {
      "key": "e_1_2_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(78)80760-1"
    },
    {
      "key": "e_1_2_3_32_2",
      "volume": "42",
      "author": "Kupke D. W.",
      "year": "1962",
      "journal-title": "Plant Physiol."
    },
    {
      "key": "e_1_2_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.47.2.252"
    },
    {
      "key": "e_1_2_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2787(74)90162-2"
    },
    {
      "key": "e_1_2_3_35_2",
      "first-page": "641",
      "volume-title": "Internartonal Symposium on Chloroplast Development",
      "author": "Edelwan M.",
      "year": "1978"
    },
    {
      "key": "e_1_2_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.64.5.828"
    },
    {
      "key": "e_1_2_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.65.4.663"
    },
    {
      "key": "e_1_2_3_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.65.4.680"
    },
    {
      "key": "e_1_2_3_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.63.2.237"
    },
    {
      "key": "e_1_2_3_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00390180"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1981.tb05486.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1981.tb05486.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T07:18:55Z",
    "timestamp": 1700723935000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1981.tb05486.x"
    }
  },
  "subtitle": [
    "Polypeptide Turnover in Darkness"
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1981,
        8
      ]
    ]
  },
  "references-count": 39,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1981,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1981.tb05486.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1981.tb05486.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1981,
        8
      ]
    ]
  }
}