DOI: 10.1111/j.1432-1033.1981.tb05319.x. Membrane‐Bound and Water‐Soluble Cytochrome c1 From Neurospora Mitochondria

Membrane‐Bound and Water‐Soluble Cytochrome c1 From Neurospora Mitochondria

10.1111/j.1432-1033.1981.tb05319.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Cytochrome c1 is a subunit of ubiquinol‐cytochrome c reductase (EC 1.10.2.2). In Neurospora crassa wild type 74A grown in the presence of chloramphenicol, the subunit is inserted only into the bilayer of the mitochondria) inner membranes without associating with other proteins. From these modified membranes a monodisperse (cytochrome c1)‐Triton complex was isolated by subjecting the Triton‐solubilized membranes to affinity chromatography on immobilized cytochrome c. A water‐soluble pentamer of cytochrome c1 was prepared from the (cytochrome c1)‐Triton complex by removing the detergent. By limited proteolytic digestion of the cytochrome c1‐Triton complex with chymotrypsin, a water‐soluble monomeric cytochrome c1 was prepared which has a molecular weight of only 24000 as compared to 3l000 of the membrane‐bound cytochrome c1. The 24000‐Mr cytochrome c1 and the 31000‐Mr cytochrome c1 have same light absorption spectra and cytochrome‐c‐binding properties. These results are used to propose the following model. Cytochrome c1 consists of a large hydrophilic part and a small hydrophobic part. The hydrophilic part extends from the mitochondrial inner membrane into the intermembrane space. This part carries the heme and interacts with cytochrome c. The hydrophobic part anchors tile cytochrome c1 to the bilayer.

Bibliography

LI, Y., LEONARD, K., & WEISS, H. (1981). MembraneâBound and WaterâSoluble Cytochrome c1 From Neurospora Mitochondria. European Journal of Biochemistry, 116(1), 199â205. Portico.

Authors 3

Yun LI (first)
Kevin LEONARD (additional)
Hanns WEISS (additional)

References 38 Referenced 64

10.1016/0304-4173(76)90012-4
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{'key': 'e_1_2_2_7_2', 'author': 'Leonard K.', 'year': '1981', 'journal-title': 'J. Mol. Biol'} / J. Mol. Biol by Leonard K. (1981)
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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 9:11 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 11:57 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 1:58 p.m.)
Issued	44 years, 4 months ago (May 1, 1981)
Published	44 years, 4 months ago (May 1, 1981)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years, 4 months ago (May 1, 1981)

Funders 0

None

BibTeX

@article{LI_1981, title={Membrane‐Bound and Water‐Soluble Cytochrome c1 From Neurospora Mitochondria}, volume={116}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05319.x}, DOI={10.1111/j.1432-1033.1981.tb05319.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={LI, Yun and LEONARD, Kevin and WEISS, Hanns}, year={1981}, month=may, pages={199–205} }

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