DOI: 10.1111/j.1432-1033.1981.tb05249.x. The ATP Substrate Site of a Cyclic‐Nucleotide‐Independent Protein Kinase from Porcine Liver Nuclei

The ATP Substrate Site of a Cyclic‐Nucleotide‐Independent Protein Kinase from Porcine Liver Nuclei

10.1111/j.1432-1033.1981.tb05249.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The ATP substrate site of a second messenger‐independent protein kinase of the type NIT from porcine liver nuclei was mapped using a series of 30 ATP derivatives with modifications at the base, ribose or triphosphate moiety. Ki values for these derivatives were determined by competition with [γ‐32P]ATP; they range from 4 μM to 1.5 mM. For a comparison with data previously reported for the catalytic subunit of cAMP‐dependent protein kinase I from rabbit skeletal muscle, the Ki values were transformed into δΔG values. These values are related to the Ki value of unsubstituted ATP and indicate the decrease of affinity caused by the different substitutions.With both enzymes the major binding affinity is derived from the interaction of the adenine base. The contributions of the two ribosyl OH groups are marginal and the triphosphate moiety interacts most strongly with its β‐phosphoryl group. Between the two enzymes the most striking differences, however, were observed for the specificity of the nucleobase interaction. While an unmodified N‐6 amino group is required in the case of the cAMP‐dependent protein kinase, the nuclear enzyme seems to tolerate extensive modification at this position, such as the introduction of a keto group or a bulky benzyl residue. Obviously, the ATP site of the nuclear kinase has an open cleft next to the N‐6 of the adenine and binding of the adenine occurs by hydrophobic interaction without the formation of hydrogen bonds to any of the adenine nitrogens.

Bibliography

BAYDOUN, H., HOPPE, J., FREIST, W., & WAGNER, K. G. (1981). The ATP Substrate Site of a CyclicâNucleotideâIndependent Protein Kinase from Porcine Liver Nuclei. European Journal of Biochemistry, 115(2), 385â389. Portico.

Authors 4

Hassan BAYDOUN (first)
Jürgen HOPPE (additional)
Wolfgang FREIST (additional)
Karl G. WAGNER (additional)

References 45 Referenced 16

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{'key': 'e_1_2_3_9_2', 'first-page': '8', 'volume-title': 'Protein Phosphorylation and Bioregulation', 'author': 'Pinna L. A.', 'year': '1980'} / Protein Phosphorylation and Bioregulation by Pinna L. A. (1980)
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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 4:06 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 9:37 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 4:41 p.m.)
Issued	44 years, 5 months ago (April 1, 1981)
Published	44 years, 5 months ago (April 1, 1981)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	44 years, 5 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{BAYDOUN_1981, title={The ATP Substrate Site of a Cyclic‐Nucleotide‐Independent Protein Kinase from Porcine Liver Nuclei}, volume={115}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05249.x}, DOI={10.1111/j.1432-1033.1981.tb05249.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BAYDOUN, Hassan and HOPPE, Jürgen and FREIST, Wolfgang and WAGNER, Karl G.}, year={1981}, month=apr, pages={385–389} }

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