DOI: 10.1111/j.1432-1033.1981.tb05232.x. Quaternary Structure of Bovine Cytochrome Oxidase

Quaternary Structure of Bovine Cytochrome Oxidase

10.1111/j.1432-1033.1981.tb05232.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A hydrodynamically homogeneous preparation of bovine mitochondrial cytochrome c oxidase can be obtained by anion‐exchange chromatography of alkaline‐treated enzyme, followed by a gel permeation chromatography step, which further removes some (aggregated) apoprotein. The molecular weight, Mr of the monodisperse enzyme in Triton X‐100 was found to be 210000. This complex is composed of six different polypeptides, with Mr summing up to about 110000 in toto, in a relative one‐to‐one stoichiometry. Two sets of these subunits constitute the 210000‐Mr enzyme complex. In contrast to our earlier report [Saraste, Penttilä, Coggins, and Wikström, FEBS Lett. 114 (1980) 35–38] the 210000‐Mr enzyme contains four (and not two) haems A, and therefore represents the dimer of cytochrome aa3. One of the proposed seven subunits, number III, is lacking in this enzyme preparation.

Bibliography

SARASTE, M., PENTTILÃ, T., & WIKSTRÃM, M. (1981). Quaternary Structure of Bovine Cytochrome Oxidase. European Journal of Biochemistry, 115(2), 261â268. Portico.

Authors 3

Matti SARASTE (first)
Timo PENTTILÄ (additional)
Mårten WIKSTRÖM (additional)

References 55 Referenced 86

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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 4:06 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 9:37 p.m.)
Indexed	1 year, 1 month ago (July 6, 2024, 1:12 a.m.)
Issued	44 years, 5 months ago (April 1, 1981)
Published	44 years, 5 months ago (April 1, 1981)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	44 years, 5 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{SARASTE_1981, title={Quaternary Structure of Bovine Cytochrome Oxidase}, volume={115}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05232.x}, DOI={10.1111/j.1432-1033.1981.tb05232.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SARASTE, Matti and PENTTILÄ, Timo and WIKSTRÖM, Mårten}, year={1981}, month=apr, pages={261–268} }

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