DOI: 10.1111/j.1432-1033.1981.tb05159.x. Cell‐Free Synthesis and Glycosylatlon of the Major Human‐Red‐Cell Sialoglycoprotein, Glycophorin A

Cell‐Free Synthesis and Glycosylatlon of the Major Human‐Red‐Cell Sialoglycoprotein, Glycophorin A

10.1111/j.1432-1033.1981.tb05159.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The human erythroid cell line, K562, synthesizes the major red cell sialoglycoprotein, glycophorin A. We have isolated an mRNA fraction which codes for glycophorin A from K562 cells and studied the synthesis of the sialoglycoprotein in a rabbit reticulocyte cell‐free system. In the absence of membranes a precursor form of glycophorin A was synthesized. This was identified using specific anti‐(glycophorin A) serum. The apparent molecular weight of the carbohydrate‐free precursor of glycophorin A wss 19500. This exceeds the molecular weight of the glycophorin A apoprotein by approximately 5000. In the presence of membranes from dog pancreas, the synthesized glycophorin A precursor was N‐glycosylated and probably also O‐glycosylated. The oligosaccharide chains remained incompleted and the glycoprotein synthesized in vitro corresponded to the glycosylated precursor of glycophorin A obtained in intact cells.

Bibliography

JOKINEN, M., ULMANEN, I., ANDERSSON, L. C., KÃÃRIÃINEN, L., & GAHMBERG, C. G. (1981). CellâFree Synthesis and Glycosylatlon of the Major HumanâRedâCell Sialoglycoprotein, Glycophorin A. European Journal of Biochemistry, 114(2), 393â397. Portico.

Authors 5

Mikko JOKINEN (first)
Ismo ULMANEN (additional)
Leif C. ANDERSSON (additional)
Leevi KÄÄRIÄINEN (additional)
Carl G. GAHMBERG (additional)

References 49 Referenced 30

10.1146/annurev.bi.45.070176.003315
10.1073/pnas.72.8.2964 / Proc. Natl Acad. Sci USA by Tomita M. (1975)
10.1021/bi00615a025
10.1084/jem.47.5.757
10.1016/0006-291X(77)90736-7
10.1007/BF00446624
10.1038/271519a0
10.1038/newbio231229a0 / Nat. New Biol. by Bretscher M. S. (1971)
10.1016/S0022-2836(75)80014-3
10.1016/0022-2836(77)90237-6
10.1016/S0021-9258(18)63563-X
10.1016/S0021-9258(18)50339-2
10.1038/279604a0
10.1016/S0021-9258(19)43774-5
10.1073/pnas.69.6.1408
10.1016/0076-6879(74)30068-7
10.1111/j.1432-1033.1976.tb10656.x
10.1111/j.1432-1033.1980.tb06108.x
10.1016/S0021-9258(17)34748-8
10.1182/blood.V52.2.379.379
10.1016/0076-6879(72)28040-5
10.1016/S0021-9258(18)63022-4
10.1084/jem.148.2.507
10.1016/S0021-9258(17)40107-4
10.1083/jcb.55.2.390
10.1016/0006-291X(72)90736-X
10.1016/S0021-9258(19)43002-0
10.1016/0076-6879(72)28094-6
10.1038/227680a0
10.1111/j.1432-1033.1974.tb03599.x
10.1002/ijc.2910230202
10.1038/278364a0
10.1016/S0021-9258(19)86009-X
10.1083/jcb.67.3.835
10.1083/jcb.67.3.852
10.1002/jss.400070308
10.1038/269775a0 / Nature (Lond.) by Rothman J. E. (1977)
10.1073/pnas.76.5.2273
10.1016/0092-8674(79)90316-7
10.1016/S0021-9258(17)34435-6
10.1016/0092-8674(77)90153-2
10.1016/0304-4157(79)90006-6
10.1016/S0021-9258(17)30047-9
10.1016/S0021-9258(17)34436-8 / J. Biol. Chem. by Kornfeld S. (1978)
10.1016/S0021-9258(17)30048-0
10.1016/S0021-9258(19)86771-6
10.1016/S0021-9258(18)35997-0
10.1016/S0021-9258(18)95872-2
10.1016/S0021-9258(19)86347-0

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 4:06 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 1:55 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 6, 2024, 8:19 a.m.)
Issued	44 years, 6 months ago (Feb. 1, 1981)
Published	44 years, 6 months ago (Feb. 1, 1981)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years, 6 months ago (Feb. 1, 1981)

Funders 0

None

BibTeX

@article{JOKINEN_1981, title={Cell‐Free Synthesis and Glycosylatlon of the Major Human‐Red‐Cell Sialoglycoprotein, Glycophorin A}, volume={114}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb05159.x}, DOI={10.1111/j.1432-1033.1981.tb05159.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={JOKINEN, Mikko and ULMANEN, Ismo and ANDERSSON, Leif C. and KÄÄRIÄINEN, Leevi and GAHMBERG, Carl G.}, year={1981}, month=feb, pages={393–397} }

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