DOI: 10.1111/j.1432-1033.1980.tb06100.x. The Protochlorophyllide Holochrome of Barley (<i>Hordeum vulgare</i> L.)

The Protochlorophyllide Holochrome of Barley (Hordeum vulgare L.)

10.1111/j.1432-1033.1980.tb06100.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The NADPH:protochlorophyllide oxidoreductase of barley has been solubilized from etioplast membranes and purified to apparent homogeneity. The highest specific activity measured for the purified enzyme was 1.6 nmol chlorophyllide formed (mg protein−1) per flash. Electrophoretic analysis of the purified enzyme on sodium dodecylsulfate/polyacrylamide gels revealed only, one polypeptide of Mr 36000. During glycerol gradient centrifugation the enzyme migrates as a low‐molecular‐weight component. It is proposed that each enzyme molecule contains only one polypeptide chain. Assuming a molecular weight of 36000 for the enzyme, it was calculated that two or three protochlorophyllide molecules are bound to each enzyme molecule.

Bibliography

APEL, K., SANTEL, H., REDLINGER, T. E., & FALK, H. (1980). The Protochlorophyllide Holochrome of Barley (Hordeum vulgare L.). European Journal of Biochemistry, 111(1), 251â258. Portico.

Authors 4

Klaus APEL (first)
Hans‐Joachim SANTEL (additional)
Tom E. REDLINGER (additional)
Heinz FALK (additional)

References 27 Referenced 169

{'key': 'e_1_2_3_2_2', 'first-page': '35', 'volume': '8', 'author': 'Boardman N. K.', 'year': '1978', 'journal-title': 'Curr. Top. Bioenerg.'} / Curr. Top. Bioenerg. by Boardman N. K. (1978)
10.1146/annurev.pp.24.060173.001021 / Annu. Rev. Plant Physiol. by Rebeiz C. A. (1973)
{'key': 'e_1_2_3_4_2', 'first-page': '299', 'volume-title': 'Comparative Biochemistry and Biophysics of Photosynthesis', 'author': 'Bogorad L.', 'year': '1968'} / Comparative Biochemistry and Biophysics of Photosynthesis by Bogorad L. (1968)
{'key': 'e_1_2_3_5_2', 'first-page': '127', 'volume-title': 'Progress in Phytochemistry', 'author': 'Harel E.', 'year': '1978'} / Progress in Phytochemistry by Harel E. (1978)
10.1016/0006-3002(62)90492-4
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{'key': 'e_1_2_3_13_2', 'first-page': '83', 'volume-title': 'Chloroplast Development', 'author': 'Dujardin E.', 'year': '1978'} / Chloroplast Development by Dujardin E. (1978)
10.1104/pp.58.1.43
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10.1016/S0021-9258(19)52451-6
10.1104/pp.43.11.1769
{'key': 'e_1_2_3_22_2', 'first-page': '33', 'volume': '10', 'author': 'Brodersen P.', 'year': '1976', 'journal-title': 'Photosynthetica (Prague)'} / Photosynthetica (Prague) by Brodersen P. (1976)
10.1016/0005-2728(71)90183-6
{'key': 'e_1_2_3_24_2', 'first-page': '375', 'volume': '6', 'author': 'Sironval C.', 'year': '1972', 'journal-title': 'Photosynthetica (Prague)'} / Photosynthetica (Prague) by Sironval C. (1972)
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{'key': 'e_1_2_3_27_2', 'first-page': '1133', 'volume': '15', 'author': 'Bovey F.', 'year': '1974', 'journal-title': 'Plant Cell Physiol'} / Plant Cell Physiol by Bovey F. (1974)
10.1093/oxfordjournals.jbchem.a126741

Dates

Type	When
Created	15 years, 1 month ago (July 21, 2010, 10:39 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:12 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 6, 2025, 8:17 a.m.)
Issued	44 years, 10 months ago (Oct. 1, 1980)
Published	44 years, 10 months ago (Oct. 1, 1980)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years, 10 months ago (Oct. 1, 1980)

Funders 0

None

BibTeX

@article{APEL_1980, title={The Protochlorophyllide Holochrome of Barley (Hordeum vulgare L.): Isolation and Characterization of the NADPH : Protochlorophyllide Oxidoreductase}, volume={111}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1980.tb06100.x}, DOI={10.1111/j.1432-1033.1980.tb06100.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={APEL, Klaus and SANTEL, Hans‐Joachim and REDLINGER, Tom E. and FALK, Heinz}, year={1980}, month=oct, pages={251–258} }

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