DOI: 10.1111/j.1432-1033.1980.tb04894.x. Isolation of Highly Active Photosystem II Particles from a Mutant of <i>Chlamydomonas reinhardtii</i>

Isolation of Highly Active Photosystem II Particles from a Mutant of Chlamydomonas reinhardtii

10.1111/j.1432-1033.1980.tb04894.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Highly active photosystem‐II particles were rapidly isolated using detergents and obtained in good yield from a mutant of the green alga Chlamydomonas reinhardtii. The particles are completely devoid of reaction centers of photosystem I, and of the secondary electron acceptor to photosystem II. They show: (a) a specific activity (ΔA of C550/unit chlorophyll) 4–7‐times that of the starting material and of spinach chloroplasts; (b) an antenna size of 40 to 50 chlorophyll molecules containing little light‐harvesting chlorophyll a/b complex (chlorophyll a/chlorophyll b= 4–6.4); (c) a ratio of variable to dark‐adapted fluorescence yield of up to 3.Further treatment of these particles by ion‐exchange chromatography largely removes five proteins and further decreases the antenna size with little loss in primary photoactivity.

Bibliography

DINER, B. A., & WOLLMAN, F. (1980). Isolation of Highly Active Photosystem II Particles from a Mutant of Chlamydomonas reinhardtii. European Journal of Biochemistry, 110(2), 521â526. Portico.

Authors 2

Bruce A. DINER (first)
Francis‐André WOLLMAN (additional)

References 25 Referenced 130

10.1016/0926-6585(66)90244-5
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10.1016/0006-291X(69)90285-X
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10.1016/0005-2728(74)90198-4
{'key': 'e_1_2_2_9_2', 'first-page': '132', 'volume': '28', 'author': 'Thornber J. P.', 'year': '1977', 'journal-title': 'Brookhaven Symp. Biol.'} / Brookhaven Symp. Biol. by Thornber J. P. (1977)
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{'key': 'e_1_2_2_15_2', 'first-page': '33', 'volume-title': 'Genetics and Biogenesis of Chloroplasts and Mitochondria', 'author': 'Bennoun P.', 'year': '1976'} / Genetics and Biogenesis of Chloroplasts and Mitochondria by Bennoun P. (1976)
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10.1016/0006-3002(60)91350-0

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 3:45 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 11:10 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 9:54 a.m.)
Issued	44 years, 11 months ago (Sept. 1, 1980)
Published	44 years, 11 months ago (Sept. 1, 1980)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years, 11 months ago (Sept. 1, 1980)

Funders 0

None

BibTeX

@article{DINER_1980, title={Isolation of Highly Active Photosystem II Particles from a Mutant of Chlamydomonas reinhardtii}, volume={110}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1980.tb04894.x}, DOI={10.1111/j.1432-1033.1980.tb04894.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={DINER, Bruce A. and WOLLMAN, Francis‐André}, year={1980}, month=sep, pages={521–526} }

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