DOI: 10.1111/j.1432-1033.1980.tb04890.x. Synthesis of a New Reagent, Ethyl 4‐azidobenzoylaminoacetimidate, and Its Use for RNA‐Protein Cross‐linking within <i>Escherichia coli</i> Ribosomal 30‐S Subunits

Synthesis of a New Reagent, Ethyl 4‐azidobenzoylaminoacetimidate, and Its Use for RNA‐Protein Cross‐linking within Escherichia coli Ribosomal 30‐S Subunits

10.1111/j.1432-1033.1980.tb04890.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A new reagent, ethyl 4‐azidobenzoylaminoacetimidate, was prepared in a four‐step synthesis starting from 4‐aminobenzoic acid. This compound was used to cross‐link RNA with proteins within the Escherichia coli 30‐S ribosomal subunits. Following the reaction of the imidoester function with protein NH2 groups, photoactivation of the azide binds the other end of the reagent to RNA. The cross‐linked proteins were labelled with 125I and identified by bidimensional gel electrophoresis. Proteins S3, S4, SS, S7, S9, S17, S18 and, in a lower and more variable yield, S12, S13, S14 and S16 were bound to 16‐S RNA. These results were confirmed by isolating cross‐linked protein‐oligonucleotide complexes from 30‐S subunits containing 32P‐labelled RNA.

Bibliography

MILLON, R., OLOMUCKI, M., LE GALL, J., GOLINSKA, B., EBEL, J., & EHRESMANN, B. (1980). Synthesis of a New Reagent, Ethyl 4âazidobenzoylaminoacetimidate, and Its Use for RNAâProtein Crossâlinking within Escherichia coli Ribosomal 30âS Subunits. European Journal of Biochemistry, 110(2), 485â492. Portico.

Authors 6

Régine MILLON (first)
Martin OLOMUCKI (additional)
Jean‐Yves LE GALL (additional)
Barbara GOLINSKA (additional)
Jean‐Pierre EBEL (additional)
Bernard EHRESMANN (additional)

References 20 Referenced 18

{'key': 'e_1_2_2_2_2', 'first-page': '89', 'volume-title': 'Ribosomes: Structure, Function and Genetics', 'author': 'Traut R. R.', 'year': '1979'} / Ribosomes: Structure, Function and Genetics by Traut R. R. (1979)
10.1016/0006-291X(73)90016-8
10.1016/0014-5793(75)80279-1
10.1016/0014-5793(75)80394-2
10.1111/j.1432-1033.1980.tb04423.x
10.1007/BF00777516
10.1007/BF00331455
10.1007/BF00693078 / Mol. Gen. Genet. by Oste C. (1977)
10.1016/0005-2787(78)90185-5
10.1007/BF00267480
10.1007/BF00267936
10.1111/j.1432-1033.1980.tb04323.x
10.1016/S0021-9258(19)42601-X
10.1111/j.1432-1033.1971.tb01584.x
10.1111/j.1432-1033.1975.tb04014.x
10.1016/0014-5793(77)80120-8
10.1111/j.1432-1033.1969.tb00733.x
10.1111/j.1432-1033.1978.tb12577.x
10.1016/0003-2697(74)90065-7
10.1016/0022-2836(79)90293-6

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 3:45 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 11:09 p.m.)
Indexed	1 year, 8 months ago (Nov. 23, 2023, 2:09 a.m.)
Issued	44 years, 11 months ago (Sept. 1, 1980)
Published	44 years, 11 months ago (Sept. 1, 1980)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years, 11 months ago (Sept. 1, 1980)

Funders 0

None

BibTeX

@article{MILLON_1980, title={Synthesis of a New Reagent, Ethyl 4‐azidobenzoylaminoacetimidate, and Its Use for RNA‐Protein Cross‐linking within Escherichia coli Ribosomal 30‐S Subunits}, volume={110}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1980.tb04890.x}, DOI={10.1111/j.1432-1033.1980.tb04890.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={MILLON, Régine and OLOMUCKI, Martin and LE GALL, Jean‐Yves and GOLINSKA, Barbara and EBEL, Jean‐Pierre and EHRESMANN, Bernard}, year={1980}, month=sep, pages={485–492} }

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