DOI: 10.1111/j.1432-1033.1980.tb04323.x. Synthesis and Properties of N‐Acetyl‐N′‐(p‐glyoxylylbenzoyl)cystamine, a New Reagent for RNA‐RNA and RNA‐Protein Cross‐Linking

Synthesis and Properties of N‐Acetyl‐N′‐(p‐glyoxylylbenzoyl)cystamine, a New Reagent for RNA‐RNA and RNA‐Protein Cross‐Linking

10.1111/j.1432-1033.1980.tb04323.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The synthesis of N‐acetyl‐N′‐(p‐glyoxylylbenzoy)cystamine (Gbz‐Cyn2‐Ac) is described. Like other glyoxal‐type reagents it reacts with guanine and arginine. The kinetics and pH dependence of these reactions are studied. (Gbz‐Cyn2‐Ac) reacts with non‐base‐paired guanines at about 20 sites in 16‐S rRNA. After reduction of disulfide bonds each derivatized guanine residue carries a free –SH group which can be used to create RNA‐RNA bridges or, after introducing an additional photoactivatable derivative, RNA‐protein bridges.

Bibliography

EXPERTâBEZANÃON, A., & HAYES, D. (1980). Synthesis and Properties of NâAcetylâNâ²â(pâglyoxylylbenzoyl)cystamine, a New Reagent for RNAâRNA and RNAâProtein CrossâLinking. European Journal of Biochemistry, 103(2), 365â375. Portico.

Authors 2

Alain EXPERT‐BEZANÇON (first)
Donal HAYES (additional)

References 33 Referenced 13

10.1016/0022-2836(76)90348-X
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 3:12 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 8:12 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 5 p.m.)
Issued	45 years, 7 months ago (Jan. 1, 1980)
Published	45 years, 7 months ago (Jan. 1, 1980)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	45 years, 7 months ago (Jan. 1, 1980)

Funders 0

None

BibTeX

@article{EXPERT_BEZAN_ON_1980, title={Synthesis and Properties of N‐Acetyl‐N′‐(p‐glyoxylylbenzoyl)cystamine, a New Reagent for RNA‐RNA and RNA‐Protein Cross‐Linking}, volume={103}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1980.tb04323.x}, DOI={10.1111/j.1432-1033.1980.tb04323.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={EXPERT‐BEZANÇON, Alain and HAYES, Donal}, year={1980}, month=jan, pages={365–375} }

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