DOI: 10.1111/j.1432-1033.1980.tb04318.x. Messenger RNA Coding for Phenylalanine Ammonia‐Lyase

Messenger RNA Coding for Phenylalanine Ammonia‐Lyase

10.1111/j.1432-1033.1980.tb04318.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The mRNA coding for phenylalanine ammonia‐lyase was partially purified from irradiated cell suspension cultures of parsley (Petroselinum hortense). The product of cell‐free translation of the mRNA in a reticulocyte lysate was isolated by immunoprecipitation and compared with the native enzyme subunit. Evidence for the identity, or at least a great similarity, of both was provided by tryptic‐peptide and gel‐electrophoretic analyses.Under partially denaturing conditions, phenylalanine ammonia‐lyase mRNA sedimented as a 20–21‐S molecule in a sucrose gradient and had an apparent molecular weight of about 1.05 × 106on a polyacrylamide gel. Approximately two‐thirds of the polynucleotide sequence of the mRNA were estimated to be required as coding sequence for the enzyme. We suggest that phenylalanine ammonia‐lyase mRNA is unlikely to code for more than one of three coordinately induced enzymes.

Bibliography

RAGG, H., & HAHLBROCK, K. (1980). Messenger RNA Coding for Phenylalanine AmmoniaâLyase. European Journal of Biochemistry, 103(2), 323â330. Portico.

Authors 2

Hermann RAGG (first)
Klaus HAHLBROCK (additional)

References 28 Referenced 10

10.1016/0003-9861(75)90364-1
10.1111/j.1432-1033.1976.tb10012.x
10.1111/j.1432-1033.1977.tb11518.x
10.1007/978-3-642-66646-9_9 / Plant Tissue Culture and Its Biotechnological Application by Hahlbrock K. (1977)
10.1016/0005-2787(77)90197-6
10.1111/j.1432-1033.1976.tb10719.x
10.1104/pp.60.3.440
10.1016/S0021-9258(17)30271-5
10.1021/bi00714a032
10.1042/bj1130131
10.1016/0076-6879(74)30066-3
10.1016/0003-9861(75)90059-4
10.1016/S0076-6879(67)12095-8 / Methods Enzymol. by McConkey E. D. (1967)
10.1515/znc-1975-3-411 / Z Naturforsch. by Gebauer H.‐U. (1975)
10.1016/S0021-9258(19)44192-6
10.1042/bj1560619 / Biochem. J. by Tilghman S. M. (1976)
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10.1016/S0021-9258(19)63365-X / J. Biol. Chem. by Atger M. (1977)
10.1016/S0021-9258(19)63338-7 / J. Biol. Chem. by Shapiro D. J. (1977)
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10.1016/0092-8674(75)90113-0
10.1016/S0021-9258(17)34828-7 / J. Biol. Chem. by Lee D. C. (1978)
10.1111/j.1432-1033.1978.tb12582.x
10.1016/B978-0-12-470205-9.50011-3

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 3:12 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 8:13 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 2:16 p.m.)
Issued	45 years, 8 months ago (Jan. 1, 1980)
Published	45 years, 8 months ago (Jan. 1, 1980)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	45 years, 8 months ago (Jan. 1, 1980)

Funders 0

None

BibTeX

@article{RAGG_1980, title={Messenger RNA Coding for Phenylalanine Ammonia‐Lyase: Characterization and Partial Purification from Cell Suspension Cultures of Petroselinum hortense}, volume={103}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1980.tb04318.x}, DOI={10.1111/j.1432-1033.1980.tb04318.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={RAGG, Hermann and HAHLBROCK, Klaus}, year={1980}, month=jan, pages={323–330} }

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