DOI: 10.1111/j.1432-1033.1979.tb13200.x. Acidic Ribosomal Proteins from Eukaryotic Cells

Acidic Ribosomal Proteins from Eukaryotic Cells

10.1111/j.1432-1033.1979.tb13200.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Precipitation of Saccharomyces cerevisiae ribosomes by ethanol under experimental conditions that do not release the ribosomal proteins can affect the activity of the particles. In the presence of 0.4 M NH4Cl and 50% ethanol only the most acidic proteins from yeast and rat liver ribosomes are released. At 1 M NH4Cl two more non‐acidic proteins are lost from the ribosomes. The release of the acidic proteins causes a small inactivation of the polymerizing activity of the particles, additional to that caused by the precipitation itself. The elongation‐factor‐2‐dependent GTP hydrolysis of the ribosomes is, however, more affected by the loss of acidic proteins. These proteins can stimulate the GTPase but not the polymerising activity when added back to the treated particles. Eukaryotic proteins cannot be sustituted for bacterial acidic proteins L7 and L12. We have not detected immunological cross‐reaction between acidic proteins from Escherichia coli and those from yeast, Artemia salina and rat liver or between acidic proteins from these eukaryotic ribosomes among themselves.

Bibliography

SÃNCHEZâMADRID, F., REYES, R., CONDE, P., & BALLESTA, J. P. G. (1979). Acidic Ribosomal Proteins from Eukaryotic Cells. European Journal of Biochemistry, 98(2), 409â416. Portico.

Authors 4

Francisco SÁNCHEZ‐MADRID (first)
Ramón REYES (additional)
Pedro CONDE (additional)
Juan P. G. BALLESTA (additional)

References 41 Referenced 94

10.1073/pnas.71.12.4723
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{'key': 'e_1_2_2_7_2', 'first-page': '524', 'volume-title': 'Lipman Sympsoium: Energy, Biosynthesis and Regulation in Molecular Biology', 'author': 'Richter D.', 'year': '1974'} / Lipman Sympsoium: Energy, Biosynthesis and Regulation in Molecular Biology by Richter D. (1974)
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 3:01 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 7:20 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 5, 2025, 8:51 a.m.)
Issued	46 years, 1 month ago (Aug. 1, 1979)
Published	46 years, 1 month ago (Aug. 1, 1979)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	46 years, 1 month ago (Aug. 1, 1979)

Funders 0

None

BibTeX

@article{S_NCHEZ_MADRID_1979, title={Acidic Ribosomal Proteins from Eukaryotic Cells: Effect on Ribosomal Functions}, volume={98}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb13200.x}, DOI={10.1111/j.1432-1033.1979.tb13200.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SÁNCHEZ‐MADRID, Francisco and REYES, Ramón and CONDE, Pedro and BALLESTA, Juan P. G.}, year={1979}, month=aug, pages={409–416} }

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