DOI: 10.1111/j.1432-1033.1979.tb13149.x. The Mode of Inhibitory Action by Aphidicolin on Eukaryotic DNA Polymerase α

The Mode of Inhibitory Action by Aphidicolin on Eukaryotic DNA Polymerase α

10.1111/j.1432-1033.1979.tb13149.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The mode of action by aphidicolin on DNA polymerase α from the nuclear fraction of seaurchin blastulae was studied. The inhibition of DNA polymerase α by aphidicolin was uncompetitive with activated DNA and competitive with the four deoxynucleoside triphosphates using activated DNA as a template‐primer. For truncated (residual or limited) DNA synthesis with only three deoxynucleoside triphosphates, aphidicolin inhibited the residual synthesis more strongly in the absence of dCTP than in the absence of each of the other three deoxynucleoside triphosphates. The inhibition was reversed with excess dCTP but not with the other three deoxynucleoside triphosphates. That is, aphidicolin inhibited DNA polymerase α by competing with dCTP with a Ki value of 0.5 μg/ml and by not competing with the other three deoxynucleoside triphosphates. dTMP incorporation with the activated DNA was more sensitive to aphidicolin than dGMP or dTMP incorporation with poly(dC) · (dG)12–18 or poly(dA) · (dT)12–18. Similar results were obtained for DNA polymerase α (B form) from mouse myeloma MOPC 104E.

Bibliography

OGURO, M., SUZUKIâHORI, C., NAGANO, H., MANO, Y., & IKEGAMI, S. (1979). The Mode of Inhibitory Action by Aphidicolin on Eukaryotic DNA Polymerase Î±. European Journal of Biochemistry, 97(2), 603â607. Portico.

Authors 5

Mieko OGURO (first)
Chiyo SUZUKI‐HORI (additional)
Hiroshi NAGANO (additional)
Yoshitake MANO (additional)
Susumu IKEGAMI (additional)

References 19 Referenced 106

10.1039/p19730002841 / J. Chem. Soc. Perkin Trans. by Dalziel W. (1973)
10.1128/AAC.4.3.294
10.1038/275458a0
10.1271/bbb1961.43.161
10.1016/0006-291X(78)90298-X
10.1016/S0021-9258(18)91737-0
10.1093/oxfordjournals.jbchem.a131857 / J. Biochem (Tokyo) by Suzuki‐Hori C. (1977)
10.1016/0005-2787(79)90486-6
{'key': 'e_1_2_2_10_2', 'first-page': '2', 'volume': '2', 'author': 'Loeb L. A.', 'year': '1977', 'journal-title': 'Pharmacol. Ther'} / Pharmacol. Ther by Loeb L. A. (1977)
10.1146/annurev.bi.46.070177.003233
10.1016/0076-6879(74)29009-8
10.1016/S0021-9258(19)52451-6
10.1016/S0021-9258(18)69770-4
10.1021/bi00676a020
10.1016/S0079-6603(08)60118-X
10.1093/nar/4.11.3981
10.1093/oxfordjournals.jbchem.a132178 / J. Biochem. (Tokyo) by Okura A. (1978)
10.1021/bi00669a017
{'key': 'e_1_2_2_20_2', 'first-page': '322', 'volume-title': 'Enzymes', 'author': 'Dixon M.', 'year': '1964'} / Enzymes by Dixon M. (1964)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 2:58 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 12:53 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 6, 2024, 2 a.m.)
Issued	46 years, 1 month ago (July 1, 1979)
Published	46 years, 1 month ago (July 1, 1979)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	46 years, 1 month ago (July 1, 1979)

Funders 0

None

BibTeX

@article{OGURO_1979, title={The Mode of Inhibitory Action by Aphidicolin on Eukaryotic DNA Polymerase α}, volume={97}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb13149.x}, DOI={10.1111/j.1432-1033.1979.tb13149.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={OGURO, Mieko and SUZUKI‐HORI, Chiyo and NAGANO, Hiroshi and MANO, Yoshitake and IKEGAMI, Susumu}, year={1979}, month=jul, pages={603–607} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        6
      ]
    ],
    "date-time": "2024-09-06T06:00:14Z",
    "timestamp": 1725602414841
  },
  "reference-count": 19,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            6,
            28
          ]
        ],
        "date-time": "2008-06-28T00:00:00Z",
        "timestamp": 1214611200000
      },
      "content-version": "vor",
      "delay-in-days": 10590,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1979,
        7
      ]
    ]
  },
  "abstract": "<jats:p>The mode of action by aphidicolin on DNA polymerase \u03b1 from the nuclear fraction of seaurchin blastulae was studied. The inhibition of DNA polymerase \u03b1 by aphidicolin was uncompetitive with activated DNA and competitive with the four deoxynucleoside triphosphates using activated DNA as a template\u2010primer. For truncated (residual or limited) DNA synthesis with only three deoxynucleoside triphosphates, aphidicolin inhibited the residual synthesis more strongly in the absence of dCTP than in the absence of each of the other three deoxynucleoside triphosphates. The inhibition was reversed with excess dCTP but not with the other three deoxynucleoside triphosphates. That is, aphidicolin inhibited DNA polymerase \u03b1 by competing with dCTP with a <jats:italic>K</jats:italic><jats:sub>i</jats:sub> value of 0.5 \u03bcg/ml and by not competing with the other three deoxynucleoside triphosphates. dTMP incorporation with the activated DNA was more sensitive to aphidicolin than dGMP or dTMP incorporation with poly(dC) \u00b7 (dG)<jats:sub>12\u201318</jats:sub> or poly(dA) \u00b7 (dT)<jats:sub>12\u201318</jats:sub>. Similar results were obtained for DNA polymerase \u03b1 (B form) from mouse myeloma MOPC 104E.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1979.tb13149.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T19:58:56Z",
    "timestamp": 1109879936000
  },
  "page": "603-607",
  "source": "Crossref",
  "is-referenced-by-count": 106,
  "title": "The Mode of Inhibitory Action by Aphidicolin on Eukaryotic DNA Polymerase \u03b1",
  "prefix": "10.1111",
  "volume": "97",
  "author": [
    {
      "given": "Mieko",
      "family": "OGURO",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Chiyo",
      "family": "SUZUKI\u2010HORI",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hiroshi",
      "family": "NAGANO",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Yoshitake",
      "family": "MANO",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Susumu",
      "family": "IKEGAMI",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        6,
        28
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_2_2_2",
      "doi-asserted-by": "crossref",
      "first-page": "2841",
      "DOI": "10.1039/p19730002841",
      "volume": "1",
      "author": "Dalziel W.",
      "year": "1973",
      "journal-title": "J. Chem. Soc. Perkin Trans."
    },
    {
      "key": "e_1_2_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/AAC.4.3.294"
    },
    {
      "key": "e_1_2_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/275458a0"
    },
    {
      "key": "e_1_2_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1271/bbb1961.43.161"
    },
    {
      "key": "e_1_2_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(78)90298-X"
    },
    {
      "key": "e_1_2_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)91737-0"
    },
    {
      "key": "e_1_2_2_8_2",
      "doi-asserted-by": "crossref",
      "first-page": "1613",
      "DOI": "10.1093/oxfordjournals.jbchem.a131857",
      "volume": "82",
      "author": "Suzuki\u2010Hori C.",
      "year": "1977",
      "journal-title": "J. Biochem (Tokyo)"
    },
    {
      "key": "e_1_2_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2787(79)90486-6"
    },
    {
      "key": "e_1_2_2_10_2",
      "first-page": "2",
      "volume": "2",
      "author": "Loeb L. A.",
      "year": "1977",
      "journal-title": "Pharmacol. Ther"
    },
    {
      "key": "e_1_2_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.46.070177.003233"
    },
    {
      "key": "e_1_2_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(74)29009-8"
    },
    {
      "key": "e_1_2_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)52451-6"
    },
    {
      "key": "e_1_2_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)69770-4"
    },
    {
      "key": "e_1_2_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00676a020"
    },
    {
      "key": "e_1_2_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0079-6603(08)60118-X"
    },
    {
      "key": "e_1_2_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/4.11.3981"
    },
    {
      "key": "e_1_2_2_18_2",
      "doi-asserted-by": "crossref",
      "first-page": "727",
      "DOI": "10.1093/oxfordjournals.jbchem.a132178",
      "volume": "84",
      "author": "Okura A.",
      "year": "1978",
      "journal-title": "J. Biochem. (Tokyo)"
    },
    {
      "key": "e_1_2_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00669a017"
    },
    {
      "key": "e_1_2_2_20_2",
      "first-page": "322",
      "volume-title": "Enzymes",
      "author": "Dixon M.",
      "year": "1964"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1979.tb13149.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1979.tb13149.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T17:53:08Z",
    "timestamp": 1700761988000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1979.tb13149.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1979,
        7
      ]
    ]
  },
  "references-count": 19,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1979,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1979.tb13149.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1979.tb13149.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1979,
        7
      ]
    ]
  }
}