DOI: 10.1111/j.1432-1033.1979.tb13074.x. DNA‐Dependent RNA Polymerase from the Archaebacterium <i>Sulfolobus acidocaldarius</i>

DNA‐Dependent RNA Polymerase from the Archaebacterium Sulfolobus acidocaldarius

10.1111/j.1432-1033.1979.tb13074.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Purified DNA‐dependent RNA polymerase from Sulfolobus acidocaldarius is composed of 10 different subunits, one of which is present as four copies. Their molecular weights are 122000, 101000, 44000, 32000, 24000, 17500, 13800, 11800 (four copies), 11200, 10800, summing up to a total Mr of 423500. The sedimentation velocity is 13.5 S, indicating that at 0.5 M NH4Cl the enzyme exists in the monomeric form. At pH 9.2 in cellogel electrophoresis two of the subunits migrate towards the cathode. The composition is quite different from that of a typical eubacterial RNA polymerase. Its complexity reminds one of eucaryotic RNA polymerase.Maximal transcription of DNA from a Halobacterium halobium phage φH (φH DNA) proceeds at pH 8.5 and 75 °C. The enzyme is stable up to 75 °C and strictly requires a DNA template. φH DNA and poly[d(A‐T) · d(A‐T)] are the most efficient. The temperature dependence of the transcription rate is characteristic for the template. Actinomycin D and heparin prevent transcription, while rifampicin, streptolydigin and α‐amanitin have no effect.During storage, even at −70°C, the enzyme loses its activity to transcribe φH DNA, whereas transcription of poly[d(A‐T) · d(A‐T)] remains unaffected.

Bibliography

ZILLIG, W., STETTER, K. O., & JANEKOVIÄ, D. (1979). DNAâDependent RNA Polymerase from the Archaebacterium Sulfolobus acidocaldarius. European Journal of Biochemistry, 96(3), 597â604. Portico.

Authors 3

Wolfram ZILLIG (first)
Karl O. STETTER (additional)
Davorin JANEKOVIĆ (additional)

References 24 Referenced 92

10.1073/pnas.74.11.5088
10.1007/BF01734485
10.1038/261669a0
10.1007/BF01796092
10.1007/BF01768019
10.1073/pnas.74.10.4537
10.1038/256505a0
10.1016/0014-5793(74)80983-X
10.1111/j.1432-1033.1978.tb20951.x
{'key': 'e_1_2_2_11_2', 'first-page': '69', 'volume-title': 'RNA Polymerase', 'author': 'Burgess R. R.', 'year': '1976'} / RNA Polymerase by Burgess R. R. (1976)
10.1007/BF00408082
10.1099/00221287-86-1-156
10.1111/j.1432-1033.1975.tb20974.x
10.1016/0014-5793(70)80519-1
10.1016/0042-6822(78)90152-6 / Virology by Stetter K. O. (1978)
10.1515/bchm2.1970.351.1.221
10.1101/SQB.1968.033.01.033
{'key': 'e_1_2_2_19_2', 'first-page': '763', 'volume-title': 'RNA Polymerase', 'author': 'Sentenac A.', 'year': '1976'} / RNA Polymerase by Sentenac A. (1976)
10.1007/BF00274183 / Mol. Gen. Genet. by Williamson V. M. (1978)
10.1515/bchm2.1977.358.2.1093 / Hoppe-Seyler's Z. Physiol. Chem. by Stetter K. O. (1977)
10.1021/bi00653a016
10.1139/m73-028
10.1126/science.170.3965.1416
Fox G. E.(1978)12th International Congress of Microbiology Munich.

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 2:57 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 4:20 p.m.)
Indexed	3 months, 1 week ago (May 18, 2025, 10:24 a.m.)
Issued	46 years, 2 months ago (June 1, 1979)
Published	46 years, 2 months ago (June 1, 1979)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	46 years, 2 months ago (June 1, 1979)

Funders 0

None

BibTeX

@article{ZILLIG_1979, title={DNA‐Dependent RNA Polymerase from the Archaebacterium Sulfolobus acidocaldarius}, volume={96}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb13074.x}, DOI={10.1111/j.1432-1033.1979.tb13074.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={ZILLIG, Wolfram and STETTER, Karl O. and JANEKOVIĆ, Davorin}, year={1979}, month=jun, pages={597–604} }

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