DOI: 10.1111/j.1432-1033.1979.tb13027.x. <i>Physarum</i> Thymidine Kinase A Step or a Peak Enzyme Depending upon Temperature of Growth

Physarum Thymidine Kinase A Step or a Peak Enzyme Depending upon Temperature of Growth

10.1111/j.1432-1033.1979.tb13027.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The variations of thymidine kinase or ATP: thymidine 5′‐phosphotransferase (EC 2.7.1.21) during the cell cycle of Physarum polycephalum plasmodia have been studied at two extreme physiological temperatures: 22°C and 32°C. At 22°C the enzyme activity increases near mitosis and stays constant during late S and G2 phases, exhibiting the typical pattern of a ‘step enzyme’. But at 32°C thymidine kinase activity goes through a maximum 1 h 30 min after mitosis and decreases during the subsequent phases as expected for a ‘peak enzyme’. The rate of enzyme degradation and/or inactivation, measured in the presence of metabolic poisons (cycloheximide or dinitrophenol), appears to follow a simple exponential function with a half‐life of approximately 3 h and 1 h at 22°C and 32°C respectively. The effect of growth temperature on the decrease of thymidine kinase activity can account entirely for the differences in the pattern of enzyme activity at the two extreme temperatures. Tentative calculations indicate that the rate of enzyme synthesis is nearly constant during the cell cycle except near mitosis, where it is temporarily increased. The results suggest the existence of a regulatory mechanism able to modulate the rate of synthesis of thymidine kinase during the cell cycle.

Bibliography

WRIGHT, M., & TOLLON, Y. (1979). Physarum Thymidine Kinase A Step or a Peak Enzyme Depending upon Temperature of Growth. European Journal of Biochemistry, 96(1), 177â181. Portico.

Authors 2

Michel WRIGHT (first)
Yvette TOLLON (additional)

References 20 Referenced 15

10.1093/oxfordjournals.aob.a090331
{'key': 'e_1_2_2_3_2', 'first-page': '43', 'volume-title': 'Methods in Cell Physiology', 'author': 'Guttes E.', 'year': '1964'} / Methods in Cell Physiology by Guttes E. (1964)
10.1016/0006-3002(60)91245-2
10.1073/pnas.53.5.924
10.1016/0006-291X(76)90900-1
10.1111/j.1432-1033.1973.tb02664.x
10.1111/j.1432-1033.1976.tb10422.x
10.1016/0014-4827(71)90227-8
{'key': 'e_1_2_2_10_2', 'first-page': '399', 'volume': '343', 'author': 'Sachsenmaier W.', 'year': '1965', 'journal-title': 'Biochem. Z.'} / Biochem. Z. by Sachsenmaier W. (1965)
10.1016/0014-5793(76)80926-X
10.2307/3573632
{'key': 'e_1_2_2_13_2', 'volume-title': 'The Biology, of the Cell Cycle', 'author': 'Mitchison J. M.', 'year': '1971'} / The Biology, of the Cell Cycle by Mitchison J. M. (1971)
10.1007/BF00268968
10.1093/genetics/66.4.623 / Genetics by Wheals A. E. (1970)
10.1016/S0006-291X(67)80085-8
10.1016/0005-2787(73)90309-2 / Biochim. Biophys. Acta by Hildebrandt A. (1973)
10.1017/S0016672300017419
10.1017/S0016672300017766 / Genet. Res. by Sudbery P. (1978)
10.1038/206093a0
10.1007/BF00267379 / Mol. gen. Genet. by Castillo L. (1978)

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 8:56 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 4:52 p.m.)
Indexed	1 year, 2 months ago (June 28, 2024, 10:07 p.m.)
Issued	46 years, 4 months ago (May 1, 1979)
Published	46 years, 4 months ago (May 1, 1979)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	46 years, 4 months ago (May 1, 1979)

Funders 0

None

BibTeX

@article{WRIGHT_1979, title={Physarum Thymidine Kinase A Step or a Peak Enzyme Depending upon Temperature of Growth}, volume={96}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb13027.x}, DOI={10.1111/j.1432-1033.1979.tb13027.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={WRIGHT, Michel and TOLLON, Yvette}, year={1979}, month=may, pages={177–181} }

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