DOI: 10.1111/j.1432-1033.1979.tb12981.x. Light‐Induced Proton Transport by Chloroplasts Suspended in Fluid Media at Sub‐Zero Temperatures: Kinetics and Stoichiometry

Light‐Induced Proton Transport by Chloroplasts Suspended in Fluid Media at Sub‐Zero Temperatures: Kinetics and Stoichiometry

10.1111/j.1432-1033.1979.tb12981.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Chloroplasts suspended in a medium containing ethanediol and water (1:1, v/v) at –16°C show light‐induced proton uptake and subsequent dark efflux. Proton uptake in continuous light showed biphasic kinetics. A 1 ms flash caused a single turnover of the photochemical centres at –16 °C. Under the same conditions 3 H+ were taken up from the external medium in the presence of methyl viologen as electron acceptor. The flash‐induced proton uptake was exponential and monophasic with t1/2= 3 s. The flash‐induced proton release into the thylakoid interior was biphasic, with half‐times of less than 0.1 s and 3 s. The fast phase represented approximately 30% of the total release and may be correlated with the oxidation of water. The half‐time of reduction of cytochrome f in the dark following illumination in the presence of 2 mM NH4Cl (2.5 s) is similar to the half‐time of the slow phase of proton release, suggesting a correlation between the kinetics of cytochrome f reduction and plastoquinol oxidation.

Bibliography

OLSEN, L. F., & COX, R. P. (1979). LightâInduced Proton Transport by Chloroplasts Suspended in Fluid Media at SubâZero Temperatures: Kinetics and Stoichiometry. European Journal of Biochemistry, 95(2), 427â432. Portico.

Authors 2

Lars F. OLSEN (first)
Raymond P. COX (additional)

References 29 Referenced 15

10.1111/j.1469-185X.1966.tb01501.x
10.1146/annurev.pp.25.060174.002231
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{'key': 'e_1_2_2_16_2', 'volume-title': 'Cryobiochemistry: An Introduction', 'author': 'Douzou P.', 'year': '1977'} / Cryobiochemistry: An Introduction by Douzou P. (1977)
10.1016/0003-2697(76)90136-6
{'key': 'e_1_2_2_18_2', 'first-page': '431', 'volume': '4', 'author': 'Hill R.', 'year': '1972', 'journal-title': 'Bioenergetics'} / Bioenergetics by Hill R. (1972)
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10.1016/S0021-9258(19)45298-8 / J. Biol. Chem. by Heath R. L. (1972)
{'key': 'e_1_2_2_26_2', 'first-page': '253', 'volume-title': 'Encyclopedia of Plant Physiology, New Series', 'author': 'Hauska G.', 'year': '1977'} / Encyclopedia of Plant Physiology, New Series by Hauska G. (1977)
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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 8:52 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 7:05 a.m.)
Indexed	11 months, 3 weeks ago (Aug. 29, 2024, 2:30 a.m.)
Issued	46 years, 4 months ago (April 1, 1979)
Published	46 years, 4 months ago (April 1, 1979)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	46 years, 4 months ago (April 1, 1979)

Funders 0

None

BibTeX

@article{OLSEN_1979, title={Light‐Induced Proton Transport by Chloroplasts Suspended in Fluid Media at Sub‐Zero Temperatures: Kinetics and Stoichiometry}, volume={95}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb12981.x}, DOI={10.1111/j.1432-1033.1979.tb12981.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={OLSEN, Lars F. and COX, Raymond P.}, year={1979}, month=apr, pages={427–432} }

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