DOI: 10.1111/j.1432-1033.1979.tb12897.x. Ribosomal Translocation Assayed by the Matrix‐Bound Poly(uridylic acid) Column Technique

Ribosomal Translocation Assayed by the Matrix‐Bound Poly(uridylic acid) Column Technique

10.1111/j.1432-1033.1979.tb12897.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The system of translation of cellulose‐bound poly(uridylic acid) by Escherichia coli ribosomes has been used for preparation of pre‐translocation state ribosomes in columns. Translocation has been induced by passing the elongation factor G (EF‐G) with GTP or its non‐cleavable analog (guanosine 5′‐[β,γ‐methylene]triphosphate) through the column. A method for quantitative comparison of translocation rates, and thus of effectiveness of translocation‐inducing factors, has been proposed. The method is based on an analysis of the profile of deacylated tRNA elution resulting from translocation in the column. The determination of the rate and amount of translocation has been done under different ionic conditions. It has been found that the Mg2+ concentration is a decisive factor of translocation in vitro: at high Mg2+ (30 mM) EF‐G cannot induce translocation, and lowering the Mg2+ concentration (to 10 mM) is required for EF‐G to become effective. Sufficiently low Mg2+ (3 mM) itself has proved to induce fast and complete translocation, without EF‐G.

Bibliography

BELITSINA, N. V., & SPIRIN, A. S. (1979). Ribosomal Translocation Assayed by the MatrixâBound Poly(uridylic acid) Column Technique. European Journal of Biochemistry, 94(1), 315â320. Portico.

Authors 2

Nadezhda V. BELITSINA (first)
Alexander S. SPIRIN (additional)

References 15 Referenced 17

{'key': 'e_1_2_2_2_2', 'first-page': '214', 'volume': '210', 'author': 'Belitsina N. V.', 'year': '1973', 'journal-title': 'Dokl. Akad. Nauk S.S.S.R.'} / Dokl. Akad. Nauk S.S.S.R. by Belitsina N. V. (1973)
{'key': 'e_1_2_2_3_2', 'first-page': '273', 'volume-title': 'Ribosomes and RNA Metabolism', 'author': 'Belitsina N. V.', 'year': '1973'} / Ribosomes and RNA Metabolism by Belitsina N. V. (1973)
10.1016/0014-5793(75)81062-3
10.1016/S0022-2836(76)80140-4
{'key': 'e_1_2_2_6_2', 'first-page': '883', 'volume': '5', 'author': 'Gavrilova L. P.', 'year': '1971', 'journal-title': 'Mol. Biol.'} / Mol. Biol. by Gavrilova L. P. (1971)
10.1016/0022-2836(76)90243-6
{'key': 'e_1_2_2_8_2', 'first-page': '1190', 'volume': '11', 'author': 'Glukhova M. A.', 'year': '1977', 'journal-title': 'Mol. Biol.'} / Mol. Biol. by Glukhova M. A. (1977)
10.1021/bi00779a026
10.1093/oxfordjournals.jbchem.a129980
10.1016/S0021-9258(19)44689-9
10.1016/S0022-2836(66)80077-3
10.1016/S0021-9258(19)42519-2 / J. Biol. Chem. by Inoue‐Yokosawa N. (1974)
{'key': 'e_1_2_2_14_2', 'first-page': '700', 'volume': '12', 'author': 'Glukhova M. A.', 'year': '1978', 'journal-title': 'Mol. Biol.'} / Mol. Biol. by Glukhova M. A. (1978)
{'key': 'e_1_2_2_15_2', 'first-page': '1467', 'volume': '179', 'author': 'Spirin A. S.', 'year': '1968', 'journal-title': 'Dokl. Akad. Nauk S.S.S.R.'} / Dokl. Akad. Nauk S.S.S.R. by Spirin A. S. (1968)
10.1101/SQB.1969.034.01.026

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 2:51 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 10:19 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 8:48 p.m.)
Issued	46 years, 6 months ago (Feb. 1, 1979)
Published	46 years, 6 months ago (Feb. 1, 1979)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	46 years, 6 months ago (Feb. 1, 1979)

Funders 0

None

BibTeX

@article{BELITSINA_1979, title={Ribosomal Translocation Assayed by the Matrix‐Bound Poly(uridylic acid) Column Technique: Effects of Different Magnesium Concentrations}, volume={94}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb12897.x}, DOI={10.1111/j.1432-1033.1979.tb12897.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BELITSINA, Nadezhda V. and SPIRIN, Alexander S.}, year={1979}, month=feb, pages={315–320} }

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