Crossref journal-article
Wiley
European Journal of Biochemistry (311)
Abstract

Polysomes specifically synthesizing the large subunit of ribulose‐1,5‐bisphosphate carboxylase were isolated from Chlamydomanas reinhardi cells by the indirect immunoprecipitation method. Electrophoretic analysis showed that the immunoprecipitated polysomes were of chloroplast origin. The mRNA coding for the large subunit which was purified from immunoprecipitated polysomes migrated at the 19‐S position on sucrose density gradients, and its molecular weight was estimated to be 7.3 × 105 by acid‐urea/agarose gel electrophoresis. The mRNA was translated in vitro with a cell‐free protein‐synthesizing system derived from Escherichia coli to give full‐length large‐subunit polypeptides.

Bibliography

SANO, H., SPAETH, E., & BURTON, W. G. (1979). Messenger RNA of the Large Subunit of Ribulose‐1,5‐Bisphosphate Carboxylase from Chlamydomonas reinhardi. European Journal of Biochemistry, 93(1), 173–180. Portico.

Authors 3
  1. Hiroshi SANO (first)
  2. Eberhard SPAETH (additional)
  3. William G. BURTON (additional)
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Dates
Type When
Created 20 years, 5 months ago (March 3, 2005, 2:43 p.m.)
Deposited 1 year, 9 months ago (Nov. 23, 2023, 12:04 a.m.)
Indexed 1 year, 9 months ago (Nov. 23, 2023, 7:39 a.m.)
Issued 46 years, 7 months ago (Jan. 1, 1979)
Published 46 years, 7 months ago (Jan. 1, 1979)
Published Online 17 years, 2 months ago (June 28, 2008)
Published Print 46 years, 7 months ago (Jan. 1, 1979)
Funders 0

None

@article{SANO_1979, title={Messenger RNA of the Large Subunit of Ribulose‐1,5‐Bisphosphate Carboxylase from Chlamydomonas reinhardi: Isolation and Properties}, volume={93}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb12808.x}, DOI={10.1111/j.1432-1033.1979.tb12808.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SANO, Hiroshi and SPAETH, Eberhard and BURTON, William G.}, year={1979}, month=jan, pages={173–180} }