DOI: 10.1111/j.1432-1033.1979.tb04227.x. Thermostable Endogenous Inhibitors of Cathepsins B and H

Thermostable Endogenous Inhibitors of Cathepsins B and H

10.1111/j.1432-1033.1979.tb04227.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Separate thermostable inhibitors of cathepsins B and H (IB and IH) were found in every rat and human tissue that was analyzed. The inhibitors were separated from the cathepsins by heating at 80–90°C at pH 3. IB and IH from rat lung were purified and found to occur in multiple molecular forms. Rat lung IB and IH were very similar proteins with molecular weights of 14000. IB and IH from hog kidney were also purified and IH was obtained free of IB. These inhibitors also displayed microheterogeneity and had molecular weights of 11000. Hog kidney IH inhibited chymopapain, but did not inhibit bromelain, chymotrypsin or cathepsin D. Rat and human serum contained thermostable inhibitors of cathepsins B and H, but these molecules had relatively high molecular weights. Therefore the low‐molecular‐weight IB and IH from other tissues appear to be intracellular in origin. When extracts of kidney or liver were autolyzed at pH 3–6, there was a large increase in cathepsin B or H activity with a concomitant decrease in IB and IH. Inhibitor destruction was apparently catalyzed by a proteinase other than cathepsin D or E. Rat kidney, liver, and spleen were rich sources of cathepsins B and H, the kidney yielding three times as much activity per gram as liver or spleen. Hog kidney was rich in cathepsin H, but yielded little or no cathepsin B. IB and IH were also present in protozoa, luna fish, chicken and toad. The inhibitors from Tetrahymena pyriformis inhibited the thiol proteinase from this protozoan.

Bibliography

LENNEY, J. F., TOLAN, J. R., SUGAI, W. J., & LEE, A. G. (1979). Thermostable Endogenous Inhibitors of Cathepsins B and H. European Journal of Biochemistry, 101(1), 153â161. Portico.

Authors 4

James F. LENNEY (first)
Jeffrey R. TOLAN (additional)
Wesley J. SUGAI (additional)
Alan G. LEE (additional)

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 2:38 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 8:56 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 9:11 a.m.)
Issued	45 years, 9 months ago (Nov. 1, 1979)
Published	45 years, 9 months ago (Nov. 1, 1979)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	45 years, 9 months ago (Nov. 1, 1979)

Funders 0

None

BibTeX

@article{LENNEY_1979, title={Thermostable Endogenous Inhibitors of Cathepsins B and H}, volume={101}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1979.tb04227.x}, DOI={10.1111/j.1432-1033.1979.tb04227.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={LENNEY, James F. and TOLAN, Jeffrey R. and SUGAI, Wesley J. and LEE, Alan G.}, year={1979}, month=nov, pages={153–161} }

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