DOI: 10.1111/j.1432-1033.1978.tb12747.x. The Poly(glycosyl) Chains of Glycoproteins.

The Poly(glycosyl) Chains of Glycoproteins.

10.1111/j.1432-1033.1978.tb12747.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Glycopeptides with complex carbohydrate structure were isolated from delipidated human erythrocyte membranes after digestion with pronase. The poly(glycosyl)peptides isolated (apparent molecular weight 4000–13 000) are suggested to contain 20–70 sugar residues in an alkali‐stable saccharide chain linked through N‐acetylglucosamine to asparagine. The main sugar components are galactose and N‐acetylglucosamine, which together account for 80% of total sugars. That the compounds isolated are glycopeptides and not glycolipids is concluded from the following findings: only trace amounts of glucose and fatty acids were present, and no long‐chain (sphingosine) bases could be detected; on the other hand, the amounts of mannose and amino acids found are compatible with an N‐glycosidic poly(glycosyl)peptide structure.The structure of the poly(glycosyl)peptides was studied using methylation analysis, exoglycosidase treatments, acid hydrolysis of the native as well as the N‐deacetylated glycopeptides, and chromium trioxide oxidation. The studies indicate that the poly(glycosyl)peptides contain a repeating ‐3)galactosyl(β1–4)N‐acetylglucosaminyl(β1‐structure with branch points at the C‐6 of the galactose residues. The saccharide chains are terminated in N‐acetylglucosaminyl, galactosyl, N‐acetylneuraminyl(α2–3 and 6)galactosyl and fucosyl(α1–2)galactosyl residues, and they also contain blood group A and B determinants.

Bibliography

KRUSIUS, T., FINNE, J., & RAUVALA, H. (1978). The Poly(glycosyl) Chains of Glycoproteins. European Journal of Biochemistry, 92(1), 289â300. Portico.

Authors 3

Tom KRUSIUS (first)
Jukka FINNE (additional)
Heikki RAUVALA (additional)

References 61 Referenced 146

{'key': 'e_1_2_3_2_2', 'volume-title': 'Membrane Glycoproteins', 'author': 'Hughes R. C.', 'year': '1976'} / Membrane Glycoproteins by Hughes R. C. (1976)
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 2:25 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 4:19 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:54 a.m.)
Issued	46 years, 8 months ago (Dec. 1, 1978)
Published	46 years, 8 months ago (Dec. 1, 1978)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	46 years, 8 months ago (Dec. 1, 1978)

Funders 0

None

BibTeX

@article{KRUSIUS_1978, title={The Poly(glycosyl) Chains of Glycoproteins.: Characterisation of a Novel Type of Glycoprotein Saccharides from Human Erythrocyte Membrane}, volume={92}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1978.tb12747.x}, DOI={10.1111/j.1432-1033.1978.tb12747.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KRUSIUS, Tom and FINNE, Jukka and RAUVALA, Heikki}, year={1978}, month=dec, pages={289–300} }

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  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1978.tb12747.x"
    }
  },
  "subtitle": [
    "Characterisation of a Novel Type of Glycoprotein Saccharides from Human Erythrocyte Membrane"
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1978,
        12
      ]
    ]
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  "references-count": 61,
  "journal-issue": {
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  "alternative-id": [
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  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1978.tb12747.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1978,
        12
      ]
    ]
  }
}