DOI: 10.1111/j.1432-1033.1978.tb12411.x. Refolding and Reactivation of Liver Alcohol Dehydrogenase after Dissociation and Denaturation in 6M Guanidine Hydrochloride

Refolding and Reactivation of Liver Alcohol Dehydrogenase after Dissociation and Denaturation in 6M Guanidine Hydrochloride

10.1111/j.1432-1033.1978.tb12411.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Horse‐liver alcohol dehydrogenase has been dissociated and denatured by 6 M gaunidinium hydrochloride. Removal of the denaturat under optimum conditions of the solvent leads to partial reactivation.The concentrations of the enzyme, as well as the coenzyme (NAD+ and Zn2+, affect the reactivation significantly, since high concentrations promote the formation of inactive aggregation products.Analyzing the kinetics of reactivation and reassociation, conditions far from equilibrium of dissociation‐association provide maximum yieldddd (∼ 70%). The sigmoidal kineic traces suggest a superposition of first‐order transconformation and‐second‐order association reactions; the latter are corrobosrated by the concentration dependence of thereactivation reaction.The coenzyme, NAD+, had no the kinetics of reactivation. Addition of Zn2+ leads to a significant decrease of the rate and yield of ractivation.The process of renaturation, as reflected by the regain of navive fluorescence show complex kinetics: rapid relaxations are followed by slower first‐order and secon‐order processes which parallel reactivtion.

Bibliography

GERSCHITZ, J., RUDOLPH, R., & JAENICKE, R. (1978). Refolding and Reactivation of Liver Alcohol Dehydrogenase after Dissociation and Denaturation in 6M Guanidine Hydrochloride. European Journal of Biochemistry, 87(3), 591â599. Portico.

Authors 3

Johan GERSCHITZ (first)
Rainer RUDOLPH (additional)
Rainer JAENICKE (additional)

References 33 Referenced 32

10.1111/j.1432-1033.1974.tb03607.x
10.1515/9783110853704-026 / 2nd Int. Symp. on Pyridine Nucleotide‐Dependent Dehydrogenases by Jaenicke R. (1977)
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 1:52 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 7:17 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 11:40 a.m.)
Issued	47 years, 1 month ago (July 1, 1978)
Published	47 years, 1 month ago (July 1, 1978)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	47 years, 1 month ago (July 1, 1978)

Funders 0

None

BibTeX

@article{GERSCHITZ_1978, title={Refolding and Reactivation of Liver Alcohol Dehydrogenase after Dissociation and Denaturation in 6M Guanidine Hydrochloride}, volume={87}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1978.tb12411.x}, DOI={10.1111/j.1432-1033.1978.tb12411.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={GERSCHITZ, Johan and RUDOLPH, Rainer and JAENICKE, Rainer}, year={1978}, month=jul, pages={591–599} }

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