DOI: 10.1111/j.1432-1033.1978.tb12337.x. Incorporation and Asymmetric Orientation of Glycophorin in Reconstituted Protein‐Containing Vesicles

Incorporation and Asymmetric Orientation of Glycophorin in Reconstituted Protein‐Containing Vesicles

10.1111/j.1432-1033.1978.tb12337.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Glycophorin can be incorporated in lipid vesicles without the use of detergents [MacDonald, R. J. and MacDonald, R. C. (1975) J. Biol. Chem. 250, 9206–9214]. In this paper it has been shown that the protein spans the lipid bilayer in these vesicles. In addition it has been shown that the protein is orientated in an asymmetric way with 75–80% of its sugar residues directed to the outside of the vesicles. Freeze‐fracturing electron microscopy suggests that the protein is present in a slightly aggregated form. Sonication of these recombinants leads to the formation of small glycophorin‐containing vesicles, in which almost all sugar residues are directed to the outside.

Bibliography

van ZOELEN, E. J. J., VERKLEIJ, A. J., ZWAAL, R. F. A., & van DEENEN, L. L. M. (1978). Incorporation and Asymmetric Orientation of Glycophorin in Reconstituted ProteinâContaining Vesicles. European Journal of Biochemistry, 86(2), 539â546. Portico.

Authors 4

Everardus J. J. van ZOELEN (first)
Arie J. VERKLEIJ (additional)
Robert F. A. ZWAAL (additional)
Laurens L. M. van DEENEN (additional)

References 37 Referenced 60

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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 1:50 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 1:08 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 2:46 a.m.)
Issued	47 years, 4 months ago (May 1, 1978)
Published	47 years, 4 months ago (May 1, 1978)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	47 years, 4 months ago (May 1, 1978)

Funders 0

None

BibTeX

@article{van_ZOELEN_1978, title={Incorporation and Asymmetric Orientation of Glycophorin in Reconstituted Protein‐Containing Vesicles}, volume={86}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1978.tb12337.x}, DOI={10.1111/j.1432-1033.1978.tb12337.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={van ZOELEN, Everardus J. J. and VERKLEIJ, Arie J. and ZWAAL, Robert F. A. and van DEENEN, Laurens L. M.}, year={1978}, month=may, pages={539–546} }

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