DOI: 10.1111/j.1432-1033.1978.tb12231.x. Interaction between Membrane Functions and Protein Synthesis in Reticulocytes: Specific Cleavage of 28‐S Ribosomal RNA by a Membrane Constituent

Interaction between Membrane Functions and Protein Synthesis in Reticulocytes: Specific Cleavage of 28‐S Ribosomal RNA by a Membrane Constituent

10.1111/j.1432-1033.1978.tb12231.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A factor isolated from rabbit reticulocyte white ghosts by Triton X‐100 treatment blocks protein synthesis at the elongation‐termination stage. Factor‐treated ribosomes were found to have an identical buoyant density to that of control ribosomes. When incubated with either reticulocyte ribosomes or ribosomal RNA, the factor products specific cuts in the 28‐S ribosomal RNA component without damaging the 18‐S RNA. Incubations of pancreatic or T1 RNase, with ribosomal RNA, at similar protein‐synthesis inhibitory concentrations effected a complete breakdown to oligo and mononucleotides.When challenged with isolated 28‐S or 18‐S reticulocyte ribosomal RNA, the highly purified factor only attacked the 28‐S RNA species. There was no accumulation of nucleotides or oligonucleotides and we concluded that the membrane factor causes inhibition of protein synthesis by having a specific endonucleolytic cleavage activity.

Bibliography

WRESCHNER, D., MELLOUL, D., & HERZBERG, M. (1978). Interaction between Membrane Functions and Protein Synthesis in Reticulocytes: Specific Cleavage of 28âS Ribosomal RNA by a Membrane Constituent. European Journal of Biochemistry, 85(1), 233â240. Portico.

Authors 3

Daniel WRESCHNER (first)
Daniele MELLOUL (additional)
Max HERZBERG (additional)

References 11 Referenced 10

10.1073/pnas.71.4.1342
10.1073/pnas.72.12.4734
10.1016/S0021-9258(17)33290-8
10.1016/S0021-9258(19)41162-9 / J. Biol. Chem. by Kabat D. (1975)
10.1111/j.1432-1033.1976.tb10315.x
10.1038/227680a0
10.1083/jcb.50.3.787
10.1016/0014-5793(71)80003-0
10.1016/0014-5793(75)80818-0
10.1016/0014-5793(77)80197-X
10.1073/pnas.69.6.1408

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 1:33 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 1:49 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 6:18 p.m.)
Issued	47 years, 5 months ago (April 1, 1978)
Published	47 years, 5 months ago (April 1, 1978)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	47 years, 5 months ago (April 1, 1978)

Funders 0

None

BibTeX

@article{WRESCHNER_1978, title={Interaction between Membrane Functions and Protein Synthesis in Reticulocytes: Specific Cleavage of 28‐S Ribosomal RNA by a Membrane Constituent}, volume={85}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1978.tb12231.x}, DOI={10.1111/j.1432-1033.1978.tb12231.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={WRESCHNER, Daniel and MELLOUL, Daniele and HERZBERG, Max}, year={1978}, month=apr, pages={233–240} }

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