DOI: 10.1111/j.1432-1033.1978.tb12163.x. Valylation of the Two RNA Components of Turnip‐Yellow Mosaic Virus and Specificity of the tRNA Aminoacylation Reaction

Valylation of the Two RNA Components of Turnip‐Yellow Mosaic Virus and Specificity of the tRNA Aminoacylation Reaction

10.1111/j.1432-1033.1978.tb12163.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A comparative study of the aminoacylation of the two RNA components of turnip yellow mosaic virus, of yeast tRNAVal, tRNAfMet and of tRNAPhe by purified yeast valyl‐tRNA synthetase is reported. Aminoacylations were performed in the presence of pure yeast tRNA nucleotidyl‐transferase, since 85% of the viral RNA molecules lacked the 3′‐adenosine. We find that aminoacylation of the viral RNAs, like tRNA aminoacylation, reflects an equilibrium between the acylation and deacylation reactions. The kinetic parameters of TYM virus RNA valylation resemble the values found for tRNAVal valylation; in particular, there is a strong affinity between the viral RNA and valyl‐tRNA synthetase and the rate constant for TYM virus RNA valylation is only slightly lower than that for tRNAVal. This result contrasts with the reduced rates observed in tRNA mischarging, and suggests that the viral RNA could be easily aminoacylated in vivo. Considering the fact that the 3′‐terminal sequence of TYM virus RNA has only a few points of resemblance to a tRNA sequence, we propose that there are some structural motifs found in both tRNAVal and TYM virus RNA which are brought in a similar spatial arrangement recognized by valyl‐tRNA synthetase.

Bibliography

GIEGÃ, R., BRIAND, J., MENGUAL, R., EBEL, J., & HIRTH, L. (1978). Valylation of the Two RNA Components of TurnipâYellow Mosaic Virus and Specificity of the tRNA Aminoacylation Reaction. European Journal of Biochemistry, 84(1), 251â256. Portico.

Authors 5

Richard GIEGÉ (first)
Jean‐Paul BRIAND (additional)
Raymond MENGUAL (additional)
Jean‐Pierre EBEL (additional)
Léon HIRTH (additional)

References 26 Referenced 58

10.1111/j.1432-1033.1974.tb03560.x
10.1016/S0300-9084(73)80415-8
10.1038/226954a0
10.1073/pnas.67.3.1345
10.1016/S0300-9084(72)80062-2
10.1016/0006-291X(72)90697-3
10.1099/0022-1317-25-2-257
10.1038/249032a0
10.1099/0022-1317-34-1-177
10.1111/j.1432-1033.1977.tb11269.x
10.1111/j.1432-1033.1977.tb11270.x
10.1093/nar/3.11.3043
10.1073/pnas.73.12.4437
10.1016/S0300-9084(76)80579-2
10.1111/j.1432-1033.1974.tb03896.x
10.1128/jvi.11.2.238-242.1973
10.1021/bi00842a023
10.1080/00327487208061475
10.1016/S0022-2836(65)80138-3
10.1111/j.1432-1033.1976.tb10965.x
10.1016/0014-5793(77)80864-8
10.1111/j.1432-1033.1972.tb02538.x
{'key': 'e_1_2_3_24_2', 'first-page': '754', 'author': 'Baev A. A.', 'year': '1967', 'journal-title': 'MoI. Biol. (Mosc.)'} / MoI. Biol. (Mosc.) by Baev A. A. (1967)
10.1016/0014-5793(71)80639-7
{'key': 'e_1_2_3_26_2', 'first-page': '508', 'volume': '49', 'author': 'Simsek M.', 'year': '1972', 'journal-title': 'Biochem. Biophys.'} / Biochem. Biophys. by Simsek M. (1972)
{'key': 'e_1_2_3_27_2', 'first-page': '143', 'volume': '127', 'author': 'Hall T. C.', 'year': '1970', 'journal-title': 'Ann. Microbiol. (Paris)'} / Ann. Microbiol. (Paris) by Hall T. C. (1970)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 1:32 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:31 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 3:55 p.m.)
Issued	47 years, 6 months ago (March 1, 1978)
Published	47 years, 6 months ago (March 1, 1978)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	47 years, 6 months ago (March 1, 1978)

Funders 0

None

BibTeX

@article{GIEG__1978, title={Valylation of the Two RNA Components of Turnip‐Yellow Mosaic Virus and Specificity of the tRNA Aminoacylation Reaction}, volume={84}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1978.tb12163.x}, DOI={10.1111/j.1432-1033.1978.tb12163.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={GIEGÉ, Richard and BRIAND, Jean‐Paul and MENGUAL, Raymond and EBEL, Jean‐Pierre and HIRTH, Léon}, year={1978}, month=mar, pages={251–256} }

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