DOI: 10.1111/j.1432-1033.1978.tb12162.x. Conformation of Methylated Sequences in HeLa Cell 18‐S Ribosomal RNA: Nuclease S<sub>1</sub> as a Probe

Conformation of Methylated Sequences in HeLa Cell 18‐S Ribosomal RNA: Nuclease S1 as a Probe

10.1111/j.1432-1033.1978.tb12162.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

18‐S rRNA from HeLa cells was digested with nuclease S1. Under the conditions employed 15% of the total nucleotides and some 50% of the methylated nucleotides were released as low‐molecular‐weight products. The material which was precipitable by 70% ethanol after nuclease S1 digestion was subjected to further digestion by combined T1 plus pancreatic riboncleases or by T1 ribonuclease alone, and fingerprints were prepared. It was found that the four sites which are modified late during ribosome maturation, and which contain base modifications, were all accessible to nuclease S1. By contrast fewer than one‐half of the sites which are modified early during ribosome maturation, and which contain 2′‐O‐methyl groups, were accessible to nuclease S1; the remainder were protected, presumably by secondary or tertiary interactions within 18‐S rRNA.

Bibliography

KHAN, M. S. N., & MADEN, B. E. H. (1978). Conformation of Methylated Sequences in HeLa Cell 18âS Ribosomal RNA: Nuclease S1 as a Probe. European Journal of Biochemistry, 84(1), 241â250. Portico.

Authors 2

M. Shah N. KHAN (first)
B. Edward H. MADEN (additional)

References 28 Referenced 13

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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 1:32 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:30 p.m.)
Indexed	1 year, 1 month ago (Aug. 3, 2024, 10:27 p.m.)
Issued	47 years, 6 months ago (March 1, 1978)
Published	47 years, 6 months ago (March 1, 1978)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	47 years, 6 months ago (March 1, 1978)

Funders 0

None

BibTeX

@article{KHAN_1978, title={Conformation of Methylated Sequences in HeLa Cell 18‐S Ribosomal RNA: Nuclease S1 as a Probe}, volume={84}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1978.tb12162.x}, DOI={10.1111/j.1432-1033.1978.tb12162.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KHAN, M. Shah N. and MADEN, B. Edward H.}, year={1978}, month=mar, pages={241–250} }

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