DOI: 10.1111/j.1432-1033.1977.tb11340.x. Ca‐Linked Phosphorylation of a Light Chain of Vertebrate Smooth‐Muscle Myosin

Ca‐Linked Phosphorylation of a Light Chain of Vertebrate Smooth‐Muscle Myosin

10.1111/j.1432-1033.1977.tb11340.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

In vertebrate smooth muscle actomyosin and myofibrils a myosin light chain of molecular weight about 20000 becomes phosphorylated at the same Ca2+ concentration as required to stimulate the actin‐activated ATPase activity of myosin. Further, the degree of phosphorylation in the preparations as well as in various reconstituted actomyosins is proportional to their measured Ca2+ sensitivity. The phosphorylation process is very rapid and is essentially completed before the rise in ATPase activity. The enzyme responsible for the observed myosin phosphorylation is a specific myosin light chain kinase which is routinely co‐purified with myosin. This kinase is normally present in actomyosin and its removal together with tropomyosin leads to a complete loss of the actin‐activated ATPase activity. It is suggested that the Ca‐dependent phosphorylation of the light chain via the light chain kinase represents the initial step in the activation of myosin that leads to contraction. Relaxation is probably effected by an as yet uncharacterised light chain phosphatase.

Bibliography

SOBIESZEK, A. (1977). CaâLinked Phosphorylation of a Light Chain of Vertebrate SmoothâMuscle Myosin. European Journal of Biochemistry, 73(2), 477â483. Portico.

Authors 1

Apolinary SOBIESZEK (first)

References 26 Referenced 145

10.1017/S0033583500001190
10.1152/physrev.1973.53.3.612 / Physiol. Rev. by Weber A. (1973)
10.1101/SQB.1973.037.01.042
10.1016/0022-2836(70)90432-8 / J. Mol. Biol. by Kendrick‐Jones J. (1970)
10.1101/SQB.1973.037.01.009
10.1038/252405a0
10.1111/j.1432-1033.1975.tb02137.x
10.1016/0022-2836(76)90074-7
Sobieszek A.&Small J. V.(1977)J. Mol. Biol.(in press).
{'key': 'e_1_2_3_11_2', 'first-page': '37', 'volume-title': 'Contractility of Muscle Cells and Related Processes', 'author': 'Weber A.', 'year': '1971'} / Contractility of Muscle Cells and Related Processes by Weber A. (1971)
{'key': 'e_1_2_3_12_2', 'first-page': '431', 'volume-title': 'Exploratory Concepts in Muscular Dystrophy II', 'author': 'Krebs E. G.', 'year': '1974'} / Exploratory Concepts in Muscular Dystrophy II by Krebs E. G. (1974)
{'key': 'e_1_2_3_13_2', 'first-page': '319', 'volume-title': 'Exploratory Concepts in Muscular Dystrophy II', 'author': 'Perry S. V.', 'year': '1974'} / Exploratory Concepts in Muscular Dystrophy II by Perry S. V. (1974)
10.1016/0014-5793(74)81232-9 / FEBS Lett. by Piers E. (1974)
10.1073/pnas.70.11.3115
{'key': 'e_1_2_3_16_2', 'first-page': '70', 'volume-title': 'Exploratory Concepts in Muscular Dystrophy II', 'author': 'Adelstein R. S.', 'year': '1974'} / Exploratory Concepts in Muscular Dystrophy II by Adelstein R. S. (1974)
10.1038/256597a0
10.1021/bi00656a019
{'key': 'e_1_2_3_19_2', 'first-page': '725', 'volume-title': 'Cell Motility', 'author': 'Adelstein R. S.', 'year': '1976'} / Cell Motility by Adelstein R. S. (1976)
10.1042/bj1510099
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10.1038/249631a0
10.1016/0022-2836(75)90094-7

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 12:38 p.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 5:58 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 4:10 p.m.)
Issued	48 years, 5 months ago (March 1, 1977)
Published	48 years, 5 months ago (March 1, 1977)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	48 years, 5 months ago (March 1, 1977)

Funders 0

None

BibTeX

@article{SOBIESZEK_1977, title={Ca‐Linked Phosphorylation of a Light Chain of Vertebrate Smooth‐Muscle Myosin}, volume={73}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1977.tb11340.x}, DOI={10.1111/j.1432-1033.1977.tb11340.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SOBIESZEK, Apolinary}, year={1977}, month=mar, pages={477–483} }

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