DOI: 10.1111/j.1432-1033.1977.tb11220.x. Lysozyme from the Insect <i>Ceratitis capitata</i> Eggs

Lysozyme from the Insect Ceratitis capitata Eggs

10.1111/j.1432-1033.1977.tb11220.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Lysozyme from eggs of the Dipterous Ceratitis capitata (Wiedeman) has been purified by ionexchange chromatography and gel filtration and its physicochemical properties have been investigated. This is the first insect lysozyme characterized so far and it exhibits some properties different to those described for other animal lysozymes. Lysozyme from the insect eggs has a molecular weight of about 23200 and a sedimentation coefficient of 2.4 S. Molecular weight determination by sodium dodecylsulphate gel electrophoresis indicates that the molecule consists of a single polypeptide chain. This lysozyme preparation shows notable stability at acidic pH values and lability at alkaline pH values. It shows a single optimum pH at about 6.5. Chitinase/muramidase specific activity ratio is around 350 times higher for the insect lysozyme than for the hen egg‐white enzyme. The amino acid composition shows the presence of one tryptophan residue per molecule of enzyme. This fact differentiates the lysozyme from insect eggs from other animal and plant lysozymes. From the amino acid composition, the absorption coefficient and the partial specific volume are calculated. Glycine is the N‐terminal residue.

Bibliography

FERNANDEZâSOUSA, J. M., GAVILANES, J. G., MUNICIO, A. M., PEREZâARANDA, A., & RODRIGUEZ, R. (1977). Lysozyme from the Insect Ceratitis capitata Eggs. European Journal of Biochemistry, 72(1), 25â33. Portico.

Authors 5

José M. FERNANDEZ‐SOUSA (first)
José G. GAVILANES (additional)
Angel M. MUNICIO (additional)
Agustin PEREZ‐ARANDA (additional)
Rosalia RODRIGUEZ (additional)

References 55 Referenced 31

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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 12:29 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 4:59 p.m.)
Indexed	2 days, 22 hours ago (Sept. 4, 2025, 10:16 a.m.)
Issued	48 years, 8 months ago (Jan. 1, 1977)
Published	48 years, 8 months ago (Jan. 1, 1977)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	48 years, 8 months ago (Jan. 1, 1977)

Funders 0

None

BibTeX

@article{FERNANDEZ_SOUSA_1977, title={Lysozyme from the Insect Ceratitis capitata Eggs}, volume={72}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1977.tb11220.x}, DOI={10.1111/j.1432-1033.1977.tb11220.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={FERNANDEZ‐SOUSA, José M. and GAVILANES, José G. and MUNICIO, Angel M. and PEREZ‐ARANDA, Agustin and RODRIGUEZ, Rosalia}, year={1977}, month=jan, pages={25–33} }

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    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1977.tb11220.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1977.tb11220.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1977,
        1
      ]
    ]
  }
}