DOI: 10.1111/j.1432-1033.1976.tb10885.x. Accumulation of Free Ribosomal PrÖteins S1, L7, and L12 in <i>Escherichia coli</i>

Accumulation of Free Ribosomal PrÖteins S1, L7, and L12 in Escherichia coli

10.1111/j.1432-1033.1976.tb10885.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The total content of free ribosomal proteins in the cells of Escherichia coli was determined to study the nature of intracellular accumulation during growth. Labeled ribosomes and post‐ribosomal supernatant were prepared from exponentially growing and stationary‐phase cultures. The fraction of free ribosomal protein in the supernatant was estimated by resolving both the acidic and basic proteins separately with two different techniques of two‐dimensional gel electrophoresis. Free ribosomal proteins in the cell sap were identified an the basis of coelectrophoresis with authentic ribosomal protein ‐markers, molecular weights and amino acid composition. Among the acidic proteins, S1, L7, and L12 Were identified and examined in detail.All three proteins accumulated to significant levels in these cultures. Stationary‐phase cells contained 2–4 times more free S1, L7, and L12 than midlogarithmic phase cells. Moreover, free S1, L7, and L12 and ribosome‐bound forms were stable during exponential and post‐exponential growth of cultures. At this growth transition, non‐ribosomal proteins in the supernatant and those associated with the ribosomes showed different characteristics of accumulation.The ratio of L12: L7 in the supematant did not exhibit a remarkable shift during the growth cycle like the ratio of L12: L7 in ribosomes. In addition, free L12 in the supernatant was not acetylation, although there was a rapid acetylation in the cells

Bibliography

RAMAGOPAL, S. (1976). Accumulation of Free Ribosomal PrÃteins S1, L7, and L12 in Escherichia coli. European Journal of Biochemistry, 69(1), 289â297. Portico.

Authors 1

Subbanaidu RAMAGOPAL (first)

References 28 Referenced 18

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10.1111/j.1432-1033.1972.tb01661.x

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 12:06 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:02 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	48 years, 10 months ago (Oct. 1, 1976)
Published	48 years, 10 months ago (Oct. 1, 1976)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	48 years, 10 months ago (Oct. 1, 1976)

Funders 0

None

BibTeX

@article{RAMAGOPAL_1976, title={Accumulation of Free Ribosomal PrÖteins S1, L7, and L12 in Escherichia coli}, volume={69}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1976.tb10885.x}, DOI={10.1111/j.1432-1033.1976.tb10885.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={RAMAGOPAL, Subbanaidu}, year={1976}, month=oct, pages={289–297} }

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