DOI: 10.1111/j.1432-1033.1976.tb10726.x. Specificity and Properties of the Destabilization, Induced by Initiation Factor IF‐3, of Ternary Complexes of the 30‐S Ribosomal Subunit, Aminoacyl‐tRNA and Polynucleotides

Specificity and Properties of the Destabilization, Induced by Initiation Factor IF‐3, of Ternary Complexes of the 30‐S Ribosomal Subunit, Aminoacyl‐tRNA and Polynucleotides

10.1111/j.1432-1033.1976.tb10726.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Initiation factor IF‐3 causes the destabilization of preformed ternary complexes of 30‐S ribosomal subunit, codons and aminoacyl‐tRNAs or peptidyl‐tRNA. This destabilization is dilution‐dependent and affects all ternary complexes with the exception of those containing the initiator fMet‐tRNA, which remain more resistant to IF‐3‐induced destabilization under the various conditions studied. Several possible reasons for this specificity have been examined. It was found that the basis for the specificity is not: (a) an intrinsic greater stability of the ternary complexes containing fMet‐tRNA, (b) the amount of aminoacyl‐tRNA bound to the ribosome, (c) the conditions under which the ternary complex is made or (d) the formylation of the amino group. On the other hand, the nature of the polynucleotide in response to which the, ternary complex is formed was found to influence the amount of aminoacyl‐tRNA bound to the ribosome, and to some extent the amount of aminoacyl‐tRNA which can be released. The ternary complex containing the mischarged initiator tRNA fVal‐tRNAfMet displays greater resistance to the IF‐3‐induced destabilization than the complex containing fVal‐tRNAVal. These results indicate that the specificity of the IF‐3 activity is due to the special structural feature of the initiator tRNA molecule and to some extent to the nature of the polynucleotide. The IF‐3‐induced destabilization of ternary complexes was found to be little affected by variations in reaction conditions, so that this IF‐3 activity can be used to measure the stoichiometric binding of IF‐3 to the ribosome over a broad range of pH and K+ and Mg2+ concentrations. Several antibiotics have been tested for their capacity to interfere with this reaction; only high concentrations of tetracycline blocked this IF‐3 activity.

Bibliography

RISULEO, G., GUALERZI, C., & PON, C. (1976). Specificity and Properties of the Destabilization, Induced by Initiation Factor IFâ3, of Ternary Complexes of the 30âS Ribosomal Subunit, AminoacylâtRNA and Polynucleotides. European Journal of Biochemistry, 67(2), 603â613. Portico.

Authors 3

Gianfranco RISULEO (first)
Claudio GUALERZI (additional)
Cynthia PON (additional)

References 30 Referenced 55

10.1016/0006-291X(71)90162-8
10.1073/pnas.71.12.4950 / Proc. Natl. Acad. Sci. U.S.A. by Pon C. L. (1974)
10.1016/0022-2836(75)90318-6 / J. Mol. Biol. by Gualerzi C. (1975)
{'key': 'e_1_2_3_5_2', 'author': 'Ewald R.', 'year': '1976', 'journal-title': 'Biochemistry'} / Biochemistry by Ewald R. (1976)
10.1038/newbio234233a0
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10.1111/j.1432-1033.1970.tb00827.x
10.1016/0005-2787(66)90261-9
10.1016/0005-2787(74)90015-X
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10.1038/newbio231126a0 / Nat. New Biol. by Eisinger J. (1971)
{'key': 'e_1_2_3_16_2', 'first-page': '166', 'volume': '43', 'author': 'Giegé R.', 'year': '1973', 'journal-title': 'FEBS Lett.'} / FEBS Lett. by Giegé R. (1973)
10.1021/bi00850a022
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10.1016/0006-291X(73)91007-3 / Biochem. Biophys. Res. Commun. by Gualerzi C. (1973)
10.1016/0022-2836(71)90255-5 / J. Mol. Biol. by White J. P. (1971)
10.1021/bi00730a022
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, noon)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:25 p.m.)
Indexed	1 year, 2 months ago (June 15, 2024, 6:34 a.m.)
Issued	49 years ago (Aug. 1, 1976)
Published	49 years ago (Aug. 1, 1976)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	49 years ago (Aug. 1, 1976)

Funders 0

None

BibTeX

@article{RISULEO_1976, title={Specificity and Properties of the Destabilization, Induced by Initiation Factor IF‐3, of Ternary Complexes of the 30‐S Ribosomal Subunit, Aminoacyl‐tRNA and Polynucleotides}, volume={67}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1976.tb10726.x}, DOI={10.1111/j.1432-1033.1976.tb10726.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={RISULEO, Gianfranco and GUALERZI, Claudio and PON, Cynthia}, year={1976}, month=aug, pages={603–613} }

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