DOI: 10.1111/j.1432-1033.1976.tb10700.x. Use of a Sequence‐Specific DNA‐Binding Ligand to Probe the Environments of <i>Eco</i>RI Restriction Endonuclease Cleavage Sites

Use of a Sequence‐Specific DNA‐Binding Ligand to Probe the Environments of EcoRI Restriction Endonuclease Cleavage Sites

10.1111/j.1432-1033.1976.tb10700.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The DNAs of bacteriophage lambda and adenovirus were incubated with the sequence‐specific DNA‐binding ligand 6,4′‐diamidino‐2‐phenylindole. Digestion of the ligand DNA complexes with EcoRI nuclease and subsequent agarose gel electrophoresis demonstrated that the ligand inhibited nuclease activity at some sites, but not at others. The results suggest that.diamidino‐2‐phenylindole can be used to probe the immediate environments of the EcoRI cleavage sites.

Bibliography

KANIA, J., & FANNING, T. G. (1976). Use of a SequenceâSpecific DNAâBinding Ligand to Probe the Environments of EcoRI Restriction Endonuclease Cleavage Sites. European Journal of Biochemistry, 67(2), 367â371. Portico.

Authors 2

Jürgen KANIA (first)
Thomas G. FANNING (additional)

References 21 Referenced 34

10.1073/pnas.72.3.784
10.1016/0092-8674(75)90018-5
10.1111/j.1432-1033.1975.tb04137.x
10.1111/j.1432-1033.1975.tb04149.x
{'key': 'e_1_2_2_6_2', 'first-page': '335', 'volume-title': 'Methods in Cell Biology', 'author': 'Williamson D.', 'year': '1975'} / Methods in Cell Biology by Williamson D. (1975)
10.1016/S0076-6879(71)21033-8 / Methods Enzymol. by Davis R. (1971)
10.1002/jlac.19717490110
10.1016/0014-5793(76)81046-0
Schreier P.(1974)Diplomarbeit Universität zu Köln pp.42.
10.1021/bi00740a018
10.1093/nar/2.10.1851 / Nucleic Acids Res. by Garfin D. (1975)
Yoshimuri R.(1971)Ph.D. Thesis University of California pp.73.
10.1016/0022-2836(75)90383-6
10.1101/SQB.1974.039.01.051
10.1016/0022-2836(69)90138-7
10.1016/S0022-2836(75)80143-4
10.1016/0022-2836(70)90219-6
10.1016/0022-2836(67)90353-1
10.1002/bip.1969.360080607
10.1016/0022-2836(70)90378-5
10.1016/0022-2836(70)90086-0

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, noon)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:22 p.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 5:24 p.m.)
Issued	49 years, 1 month ago (Aug. 1, 1976)
Published	49 years, 1 month ago (Aug. 1, 1976)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	49 years, 1 month ago (Aug. 1, 1976)

Funders 0

None

BibTeX

@article{KANIA_1976, title={Use of a Sequence‐Specific DNA‐Binding Ligand to Probe the Environments of EcoRI Restriction Endonuclease Cleavage Sites}, volume={67}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1976.tb10700.x}, DOI={10.1111/j.1432-1033.1976.tb10700.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KANIA, Jürgen and FANNING, Thomas G.}, year={1976}, month=aug, pages={367–371} }

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