DOI: 10.1111/j.1432-1033.1976.tb10639.x. The Energetics of <i>Escherichia coli</i> during Aerobic Growth in Continuous Culture

The Energetics of Escherichia coli during Aerobic Growth in Continuous Culture

10.1111/j.1432-1033.1976.tb10639.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The energetics of Escherichia coli W growing aerobically in continuous culture have been investigated. Conditions were chosen such that growth was limited by the availability of carbon or oxygen (energy‐limited cultures), or of ammonium or sulphate ions (excess energy cultures). Under glycerol‐limited conditions YmaxO2(true molar growth yield with respect to oxygen) and YmaxATP (true molar growth yield with respect to ATP equivalents) were 50.9 g cells · mol O2−1 and 12.7 g cells mol ATP equivalents−1 respectively; these values were not substantially altered during growth limited by oxygen. ammonium or sulphate. In contrast, M (the energy requirement. for maintenance purposes) increased from approximately 2 mmol ATP equivalents · h−1 g.cell−1 during energy‐limited growth to 16.8 and 30.8 mmole ATP equivalents h−1 g cells−1 when growth was limited by ammonium and sulphate ions respectively. Replacement of glycerol by other limiting carbon sources caused YmaxATP to alter within the range 13.9 (glucose) to 7.1 (acetate) g cells mol ATP equivalents−1 in the order glucose > galactose > arabinose > fructose > glycerol > fumarate > lactate > pyruvate > acetate. In each case the experimental value of YmaxATP was ≤ 55% of the theoretical value calculated from the known energy requirements for the biosynthesis of cell materials. It is concluded from these results that neither M nor YmaxATP are constant values for E. coli. M varies with the energy supply, being highest under excess energy growth conditions where it may reflect energy wastage by the cell. On the other hand, YmaxATP varies with the nature of the growth substrate and thus reflects the different energy requirements for the synthesis of cell material from different carbon sources

Bibliography

FARMER, I. S., & JONES, C. W. (1976). The Energetics of Escherichia coli during Aerobic Growth in Continuous Culture. European Journal of Biochemistry, 67(1), 115â122. Portico.

Authors 2

Ian S. FARMER (first)
Colin W. JONES (additional)

References 50 Referenced 70

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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 11:58 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 1:11 p.m.)
Indexed	3 months, 4 weeks ago (May 6, 2025, 1:08 p.m.)
Issued	49 years, 1 month ago (Aug. 1, 1976)
Published	49 years, 1 month ago (Aug. 1, 1976)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	49 years, 1 month ago (Aug. 1, 1976)

Funders 0

None

BibTeX

@article{FARMER_1976, title={The Energetics of Escherichia coli during Aerobic Growth in Continuous Culture}, volume={67}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1976.tb10639.x}, DOI={10.1111/j.1432-1033.1976.tb10639.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={FARMER, Ian S. and JONES, Colin W.}, year={1976}, month=aug, pages={115–122} }

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  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1976.tb10639.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1976,
        8
      ]
    ]
  }
}