DOI: 10.1111/j.1432-1033.1976.tb10027.x. The Reduction of Plastocyanin by Plastoquinol‐1 in the Presence of Chloroplasts

The Reduction of Plastocyanin by Plastoquinol‐1 in the Presence of Chloroplasts

10.1111/j.1432-1033.1976.tb10027.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The reduction of plastocyanin by plastoquinol‐1 was efficiently catalysed by disrupted chioroplasts or etioplasts in the dark. The reaction was inhibited by 2,5‐dibromomethylisopropyl‐p‐benzoquinone which inhibits photosynthetic electron transport between plastoquinone and cytochrome f. Evidence is presented that the reduction took place via cytochrome f, and that plastoquinone‐9 was not involved. Triton X‐100 and organic solvents were inhibitory, but partial fractionation was achieved without loss of activity by density gradient centrifugation in the presence of high digitonin concentrations. All active material contained cytochromes b‐559LP and b‐563 in addition to cytochrome f, but these b‐type cytochromes were not directly involved. Other 1‐electron acceptors could be used in place of plastocyanin, for instance ferricyanide and Pseudomonas cytochrome c‐551. The reaction can be applied to give a sensitive dark assay for active cytochrome f It is suggested that cytochrome f possesses two sites for interaction with redox reagents: a hydrophilic site with which plastocyanin reacts by electron transfer and a hydrophobic site with which plastoquinol reacts by hydrogen atom transfer.

Bibliography

WOOD, P. M., & BENDALL, D. S. (1976). The Reduction of Plastocyanin by Plastoquinolâ1 in the Presence of Chloroplasts. European Journal of Biochemistry, 61(2), 337â344. Portico.

Authors 2

P. Muir WOOD (first)
Derek S. BENDALL (additional)

References 47 Referenced 79

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{'key': 'e_1_2_2_32_2', 'first-page': '175', 'volume-title': 'Biochemistry of Quinones', 'author': 'Redfearn E. R.', 'year': '1965'} / Biochemistry of Quinones by Redfearn E. R. (1965)
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:28 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 2:27 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 9:15 a.m.)
Issued	49 years, 7 months ago (Jan. 1, 1976)
Published	49 years, 7 months ago (Jan. 1, 1976)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	49 years, 7 months ago (Jan. 1, 1976)

Funders 0

None

BibTeX

@article{WOOD_1976, title={The Reduction of Plastocyanin by Plastoquinol‐1 in the Presence of Chloroplasts: A Dark Electron Transfer Reaction Involving Components between the Two Photosystems}, volume={61}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1976.tb10027.x}, DOI={10.1111/j.1432-1033.1976.tb10027.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={WOOD, P. Muir and BENDALL, Derek S.}, year={1976}, month=jan, pages={337–344} }

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