DOI: 10.1111/j.1432-1033.1975.tb04165.x. 12‐(9‐Anthroyl)stearic Acid, a Fluorescent Probe for the Ubiquinone Region of the Mitochondrial Membrane

12‐(9‐Anthroyl)stearic Acid, a Fluorescent Probe for the Ubiquinone Region of the Mitochondrial Membrane

10.1111/j.1432-1033.1975.tb04165.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

12‐(9‐Anthroyl)stearic acid can be incorporated into mitochondrial membranes. The fluorescence properties of the membrane‐bound probe are different from those of the free molecule. The intensity of emission and fluorescence life‐time of the probe is enhanced when, in the presence of substrate, the electron‐transport chain is reduced. This change in intensity has been demonstrated to be a result of collisional quenching by oxidised ubiquinone in the oxidised membrane but not when the respiratory chain is in the reduced state. In pulsing anaerobic mitochondria with oxygen the rate of the fluorescence change is found to be slower than the rate of ubiquinone oxidation, suggesting that the probe detects a structural transition in the mitochondrial inner membrane. This transition results in a constraint on ubiquinone motion in the reduced system. Model experiments, using lipid dispersions, have been carried out to test some of the interpretations.

Bibliography

CHANCE, B., ERECINSKA, M., & RADDA, G. K. (1975). 12â(9âAnthroyl)stearic Acid, a Fluorescent Probe for the Ubiquinone Region of the Mitochondrial Membrane. European Journal of Biochemistry, 54(2), 521â529. Portico.

Authors 3

Britton CHANCE (first)
Maria ERECINSKA (additional)
George K. RADDA (additional)

References 26 Referenced 38

10.1016/B978-0-12-152504-0.50009-5
{'key': 'e_1_2_1_3_2', 'volume-title': 'Probes of Structure and Function of Macromolecules and Membrane', 'author': 'Chance B.', 'year': '1971'} / Probes of Structure and Function of Macromolecules and Membrane by Chance B. (1971)
10.1073/pnas.62.2.612
10.1042/bj1160721
10.1042/bj1220385
10.1073/pnas.69.6.1499
10.1016/0005-2736(71)90043-5
10.1073/pnas.67.2.579
{'key': 'e_1_2_1_10_2', 'first-page': '3', 'volume-title': 'Proc. Vth Int. Cong. Biochem.', 'author': 'Chance B.', 'year': '1963'} / Proc. Vth Int. Cong. Biochem. by Chance B. (1963)
10.1016/S0021-9258(18)56449-8
10.1021/bi00760a031
10.1016/0006-3002(61)90297-9
10.1016/0005-2728(72)90055-2
{'key': 'e_1_2_1_15_2', 'first-page': '674', 'volume': '338', 'author': 'Szarkowska L.', 'year': '1963', 'journal-title': 'Biochem. Z.'} / Biochem. Z. by Szarkowska L. (1963)
{'key': 'e_1_2_1_16_2', 'first-page': '437', 'volume-title': 'Fast Reactions and Primary Processes in Chemical Kinetics', 'author': 'Chance B.', 'year': '1967'} / Fast Reactions and Primary Processes in Chemical Kinetics by Chance B. (1967)
10.1073/pnas.66.4.1175
10.1016/S0021-9258(18)57021-6
10.1073/pnas.69.1.50
10.1016/0014-5793(72)80601-X
{'key': 'e_1_2_1_21_2', 'first-page': '335', 'volume': '338', 'author': 'Green D. E.', 'year': '1963', 'journal-title': 'Biochem. Z.'} / Biochem. Z. by Green D. E. (1963)
{'key': 'e_1_2_1_22_2', 'first-page': '459', 'volume-title': 'Biochemistry of Quinones', 'author': 'Chance B.', 'year': '1965'} / Biochemistry of Quinones by Chance B. (1965)
{'key': 'e_1_2_1_23_2', 'first-page': '85', 'volume-title': 'Biochemistry and Biophysics of Mitochondrial Membranes', 'author': 'Chance B.', 'year': '1972'} / Biochemistry and Biophysics of Mitochondrial Membranes by Chance B. (1972)
10.1016/0005-2760(72)90085-9
{'key': 'e_1_2_1_25_2', 'first-page': '441', 'volume-title': 'Photophysics of Aromatic Molecules', 'author': 'Birks J. B.', 'year': '1970'} / Photophysics of Aromatic Molecules by Birks J. B. (1970)
{'key': 'e_1_2_1_26_2', 'first-page': '1978', 'volume': '178', 'author': 'Perrin F.', 'year': '1924', 'journal-title': 'C. R. Hebd. Séances Acad. Sci. (Paris)'} / C. R. Hebd. Séances Acad. Sci. (Paris) by Perrin F. (1924)
{'key': 'e_1_2_1_27_2', 'first-page': '183', 'volume': '20', 'author': 'Stern O.', 'year': '1919', 'journal-title': 'Physik. Z.'} / Physik. Z. by Stern O. (1919)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:21 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 12:41 p.m.)
Issued	50 years, 2 months ago (June 1, 1975)
Published	50 years, 2 months ago (June 1, 1975)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	50 years, 2 months ago (June 1, 1975)

Funders 0

None

BibTeX

@article{CHANCE_1975, title={12‐(9‐Anthroyl)stearic Acid, a Fluorescent Probe for the Ubiquinone Region of the Mitochondrial Membrane}, volume={54}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1975.tb04165.x}, DOI={10.1111/j.1432-1033.1975.tb04165.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={CHANCE, Britton and ERECINSKA, Maria and RADDA, George K.}, year={1975}, month=jun, pages={521–529} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T17:41:09Z",
    "timestamp": 1700761269874
  },
  "reference-count": 26,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            6,
            28
          ]
        ],
        "date-time": "2008-06-28T00:00:00Z",
        "timestamp": 1214611200000
      },
      "content-version": "vor",
      "delay-in-days": 12081,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1975,
        6
      ]
    ]
  },
  "abstract": "<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>12\u2010(9\u2010Anthroyl)stearic acid can be incorporated into mitochondrial membranes.</jats:p></jats:list-item>\n<jats:list-item><jats:p>The fluorescence properties of the membrane\u2010bound probe are different from those of the free molecule.</jats:p></jats:list-item>\n<jats:list-item><jats:p>The intensity of emission and fluorescence life\u2010time of the probe is enhanced when, in the presence of substrate, the electron\u2010transport chain is reduced.</jats:p></jats:list-item>\n<jats:list-item><jats:p>This change in intensity has been demonstrated to be a result of collisional quenching by oxidised ubiquinone in the oxidised membrane but not when the respiratory chain is in the reduced state.</jats:p></jats:list-item>\n<jats:list-item><jats:p>In pulsing anaerobic mitochondria with oxygen the rate of the fluorescence change is found to be slower than the rate of ubiquinone oxidation, suggesting that the probe detects a structural transition in the mitochondrial inner membrane.</jats:p></jats:list-item>\n<jats:list-item><jats:p>This transition results in a constraint on ubiquinone motion in the reduced system. Model experiments, using lipid dispersions, have been carried out to test some of the interpretations.</jats:p></jats:list-item>\n</jats:list>\n</jats:p>",
  "DOI": "10.1111/j.1432-1033.1975.tb04165.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T16:00:23Z",
    "timestamp": 1109865623000
  },
  "page": "521-529",
  "source": "Crossref",
  "is-referenced-by-count": 38,
  "title": "12\u2010(9\u2010Anthroyl)stearic Acid, a Fluorescent Probe for the Ubiquinone Region of the Mitochondrial Membrane",
  "prefix": "10.1111",
  "volume": "54",
  "author": [
    {
      "given": "Britton",
      "family": "CHANCE",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Maria",
      "family": "ERECINSKA",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "George K.",
      "family": "RADDA",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        6,
        28
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-152504-0.50009-5"
    },
    {
      "key": "e_1_2_1_3_2",
      "volume-title": "Probes of Structure and Function of Macromolecules and Membrane",
      "author": "Chance B.",
      "year": "1971"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.62.2.612"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1160721"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1220385"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.69.6.1499"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(71)90043-5"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.67.2.579"
    },
    {
      "key": "e_1_2_1_10_2",
      "first-page": "3",
      "volume-title": "Proc. Vth Int. Cong. Biochem.",
      "author": "Chance B.",
      "year": "1963"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)56449-8"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00760a031"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-3002(61)90297-9"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(72)90055-2"
    },
    {
      "key": "e_1_2_1_15_2",
      "first-page": "674",
      "volume": "338",
      "author": "Szarkowska L.",
      "year": "1963",
      "journal-title": "Biochem. Z."
    },
    {
      "key": "e_1_2_1_16_2",
      "first-page": "437",
      "volume-title": "Fast Reactions and Primary Processes in Chemical Kinetics",
      "author": "Chance B.",
      "year": "1967"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.66.4.1175"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)57021-6"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.69.1.50"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(72)80601-X"
    },
    {
      "key": "e_1_2_1_21_2",
      "first-page": "335",
      "volume": "338",
      "author": "Green D. E.",
      "year": "1963",
      "journal-title": "Biochem. Z."
    },
    {
      "key": "e_1_2_1_22_2",
      "first-page": "459",
      "volume-title": "Biochemistry of Quinones",
      "author": "Chance B.",
      "year": "1965"
    },
    {
      "key": "e_1_2_1_23_2",
      "first-page": "85",
      "volume-title": "Biochemistry and Biophysics of Mitochondrial Membranes",
      "author": "Chance B.",
      "year": "1972"
    },
    {
      "key": "e_1_2_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2760(72)90085-9"
    },
    {
      "key": "e_1_2_1_25_2",
      "first-page": "441",
      "volume-title": "Photophysics of Aromatic Molecules",
      "author": "Birks J. B.",
      "year": "1970"
    },
    {
      "key": "e_1_2_1_26_2",
      "first-page": "1978",
      "volume": "178",
      "author": "Perrin F.",
      "year": "1924",
      "journal-title": "C. R. Hebd. S\u00e9ances Acad. Sci. (Paris)"
    },
    {
      "key": "e_1_2_1_27_2",
      "first-page": "183",
      "volume": "20",
      "author": "Stern O.",
      "year": "1919",
      "journal-title": "Physik. Z."
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1975.tb04165.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1975.tb04165.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T11:21:42Z",
    "timestamp": 1700738502000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1975.tb04165.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1975,
        6
      ]
    ]
  },
  "references-count": 26,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1975,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1975.tb04165.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1975.tb04165.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1975,
        6
      ]
    ]
  }
}