DOI: 10.1111/j.1432-1033.1975.tb04120.x. The Mitochondrial ATPase

10.1111/j.1432-1033.1975.tb04120.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Evidence is presented which indicates that inactivation of the mitochondrial ATPase from bovine heart by the reagent 4‐chloro‐7‐nitrobenzofurazan results from modification of one tyrosine residue per enzyme molecule. Activity can be restored by a variety of sulphydryl reagents. In sodium dodecyl sulphate, the nitrobenzofurazan group on tyrosine is transferred to newly exposed sulphydryl groups on the enzyme. The rate of transfer of the nitrobenzofurazan moiety from the enzyme to sulphydryl compounds is compared with that for transfer from the model compound N‐acetyl‐tyrosine‐O(7‐nitrobenzofurazan) ethyl ester, the synthesis and properties of which are also described. The ligands ATP and ADP exert a protective effect on the rate of reaction between the mitochondrial ATPase and 4‐chloro‐7‐nitrobenzofurazan. The variation in rate of this reaction with change in pH has also been examined and a pKa of 9.5 estimated for the tyrosine residue. The modification does not prevent substrate binding as judged by changes in the fluorescence of aurovertin, an antibiotic with specific affinity for mitochondrial ATPases. When the ATPase activity of submitochondrial particles is inhibited by 4‐chloro‐7‐nitrobenzofurazan, there is a parallel decrease in the extent of the energy‐linked fluorescence enhancement of 1‐anilino‐naphthalene‐8‐sulphonate induced by ATP hydrolysis. Both ATPase activity and the fluorescence enhancement are restored by sulphydryl reagents.

Bibliography

FERGUSON, S. J., LLOYD, W. J., LYONS, M. H., & RADDA, G. K. (1975). The Mitochondrial ATPase. European Journal of Biochemistry, 54(1), 117â126. Portico.

Authors 4

Stuart J. FERGUSON (first)
William J. LLOYD (additional)
Michael H. LYONS (additional)
George K. RADDA (additional)

References 33 Referenced 167

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{'key': 'e_1_2_3_21_2', 'first-page': '88', 'volume': '27', 'author': 'Baldwin C. L.', 'year': '1964', 'journal-title': 'Lloydia (Cinci.)'} / Lloydia (Cinci.) by Baldwin C. L. (1964)
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10.1111/j.1432-1033.1975.tb04121.x
{'key': 'e_1_2_3_34_2', 'first-page': '602', 'volume': '33', 'author': 'Deters D.', 'year': '1974', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Deters D. (1974)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 1:02 p.m.)
Deposited	1 year, 8 months ago (Nov. 23, 2023, 3:26 a.m.)
Indexed	5 hours, 40 minutes ago (Aug. 22, 2025, 12:46 a.m.)
Issued	50 years, 3 months ago (May 1, 1975)
Published	50 years, 3 months ago (May 1, 1975)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	50 years, 3 months ago (May 1, 1975)

Funders 0

None

BibTeX

@article{FERGUSON_1975, title={The Mitochondrial ATPase: Evidence for a Single Essential Tyrosine Residue}, volume={54}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1975.tb04120.x}, DOI={10.1111/j.1432-1033.1975.tb04120.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={FERGUSON, Stuart J. and LLOYD, William J. and LYONS, Michael H. and RADDA, George K.}, year={1975}, month=may, pages={117–126} }

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