DOI: 10.1111/j.1432-1033.1975.tb04113.x. Metabolism of the Phosphatidylglycerol Molecular Species in <i>Escherichia coli</i>

Metabolism of the Phosphatidylglycerol Molecular Species in Escherichia coli

10.1111/j.1432-1033.1975.tb04113.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The fractionation, turnover and biosynthesis of the phosphatidylglycerol molecular species of Escherichia coli were studied. Monoacetyldiglycerides derived from phosphatidylglycerol were separated into five subfractions; cis‐vaccenyl‐palmitoleyl, cis‐vaccenyl‐cis‐vaccenyl, palmityl‐palmitoleyl, palmityl‐cis‐vaccenyl and the disaturated molecular species on a silica gel plate impregnated with silver nitrate. Individual molecular species had different turnover rates. The palmityl‐cis‐vaccenyl species was metabolized faster than the others. Disaturated species were rather stable. Various phosphatidylglycerol molecular species were synthesized in the presence of sn‐glycerol 3‐phosphate, palmityl‐CoA, palmitoleyl‐CoA, cis‐vaccenyl‐CoA and CTP by the E. coli membrane particulate fraction. When only the proportion of palmityl‐CoA among the three acyl‐CoAs was increased, the molecular species containing the palmityl residue were increased. Similar results were obtained with the other acyl‐CoAs. However, a temperature‐sensitive incorporation of unsaturated and saturated fatty acids into phosphatidylglycerol molecular species was observed with no change in the proportions of the three acyl‐CoAs, completely reflecting the in vivo unsaturated/saturated ratio.

Bibliography

KITO, M., ISHINAGA, M., NISHIHARA, M., KATO, M., HATA, T., & SAWADA, S. (1975). Metabolism of the Phosphatidylglycerol Molecular Species in Escherichia coli. European Journal of Biochemistry, 54(1), 55â63. Portico.

Authors 6

Makoto KITO (first)
Masataka ISHINAGA (additional)
Masateru NISHIHARA (additional)
Michie KATO (additional)
Tadao HATA (additional)
Seiji SAWADA (additional)

References 43 Referenced 64

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 1:02 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 3:28 a.m.)
Indexed	1 year, 7 months ago (Jan. 16, 2024, 11:21 a.m.)
Issued	50 years, 3 months ago (May 1, 1975)
Published	50 years, 3 months ago (May 1, 1975)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	50 years, 3 months ago (May 1, 1975)

Funders 0

None

BibTeX

@article{KITO_1975, title={Metabolism of the Phosphatidylglycerol Molecular Species in Escherichia coli}, volume={54}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1975.tb04113.x}, DOI={10.1111/j.1432-1033.1975.tb04113.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KITO, Makoto and ISHINAGA, Masataka and NISHIHARA, Masateru and KATO, Michie and HATA, Tadao and SAWADA, Seiji}, year={1975}, month=may, pages={55–63} }

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