DOI: 10.1111/j.1432-1033.1975.tb04013.x. The Specificity of Five DNAases as Studied by the Analysis of 5′‐Terminal Doublets

The Specificity of Five DNAases as Studied by the Analysis of 5′‐Terminal Doublets

10.1111/j.1432-1033.1975.tb04013.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The 5′‐terminal dinucleotides released by five deoxyribonucleases (spleen acid DNase, snail acid DNase, pancreatic DNase, Escherichia coli endonuclease I and crab DNase) have been determined on E. coli DNA (51% dG + dC) digests having different average sizes (P̄n) in the range 50 to 10. It has been shown that the composition of the 5′‐terminal dinucleotide (a) is independent upon the degradation level, at least in the range explored; (b) is strongly different from the composition of E. coli DNA doublets, these differences being characteristic for each enzyme; (c) is very significantly different from the statistical composition of 5′‐terminal dinucleotides, as calculated from the composition of 5′‐terminal and penultimate nucleotides. A calculation of the statistical composition of the trinucleotides split by each enzyme, using the 3′‐terminal nucleotide data in conjunction with the 5′‐terminal dinucleotide results provided a qualitative “specificity spectrum” for each enzyme.

Bibliography

BERNARDI, A., GAILLARD, C., & BERNARDI, G. (1975). The Specificity of Five DNAases as Studied by the Analysis of 5â²âTerminal Doublets. European Journal of Biochemistry, 52(3), 451â457. Portico.

Authors 3

Alberto BERNARDI (first)
Claire GAILLARD (additional)
Giorgio BERNARDI (additional)

References 16 Referenced 27

10.1038/newbio246036a0
{'key': 'e_1_2_3_3_2', 'author': 'Bernardi A.', 'year': '1975', 'journal-title': 'Anal. Biochem.'} / Anal. Biochem. by Bernardi A. (1975)
10.1021/bi00898a005
10.1021/bi00769a016
10.1016/S0021-9258(18)60378-3
10.1021/bi00713a001 / Biochemistry. by Sabeur G. (1974)
10.1111/j.1432-1033.1975.tb04012.x / Eur. J. Biochem. by Sdbeur G. (1975)
10.1111/j.1432-1033.1973.tb03077.x
10.1016/0005-2787(66)90002-5
10.1111/j.1432-1033.1973.tb03076.x
10.1111/j.1432-1033.1973.tb03176.x / Eur. J. Biochem. by Laval J. (1973)
10.1111/j.1432-1033.1973.tb03178.x
10.1111/j.1432-1033.1973.tb03179.x
10.1016/S0021-9258(18)64321-2
10.1111/j.1432-1033.1973.tb03177.x / Eur. J. Biochem. by Ehrlich S. D. (1973)
10.1093/nar/1.1.87 / Nucleic Acids Res. by Ehrlich S. D. (1974)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 10:55 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 9:16 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:19 a.m.)
Issued	50 years, 4 months ago (April 1, 1975)
Published	50 years, 4 months ago (April 1, 1975)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	50 years, 4 months ago (April 1, 1975)

Funders 0

None

BibTeX

@article{BERNARDI_1975, title={The Specificity of Five DNAases as Studied by the Analysis of 5′‐Terminal Doublets}, volume={52}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1975.tb04013.x}, DOI={10.1111/j.1432-1033.1975.tb04013.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BERNARDI, Alberto and GAILLARD, Claire and BERNARDI, Giorgio}, year={1975}, month=apr, pages={451–457} }

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