DOI: 10.1111/j.1432-1033.1975.tb03906.x. Protein A from <i>Staphylococcus aureus</i>

10.1111/j.1432-1033.1975.tb03906.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Protein A, a cell‐wall protein from Staphylococcus aureus, has been studied by spectrophotometry and spectropolarimetry. All the four tyrosines are similarily titrated with pKa= 10.25. Circular dichroism (CD) spectra show that the conformation of protein A is very stable over a large pH interval (0.99–11.8). The conformation is partly intact even in 6 M guanidine hydrochloride and at 80°C. Protein A contains about 50%α‐helical structure and 10–20%β‐structures. CD band maxima at 261 and 268 nm are ascribed to transitions in the phenylalanine residues and ellipticities between 275–285 nm to the tyrosine residues. Of the four tyrosines, 3.5 are perturbed by 20% polyethylene glycol, while all of them are perturbed by 20% dimethylsulfoxide. The role of the tyrosines in the reaction with the Fc‐region of immunoglobulins is discussed.

Bibliography

SJÃHOLM, I. (1975). Protein A from Staphylococcus aureus. European Journal of Biochemistry, 51(1), 55â61. Portico.

Authors 1

Ingvar SJÖHOLM (first)

References 25 Referenced 50

10.1111/j.1699-0463.1958.tb01093.x
10.4049/jimmunol.97.6.822
10.1111/j.1432-1033.1972.tb02023.x
Forsgren A.(1968)Acta Univ. Upsaliensis56(thesis).
10.4049/jimmunol.104.1.140
10.4049/jimmunol.99.1.19
10.1111/j.1432-1033.1972.tb02024.x
10.4049/jimmunol.110.6.1562 / J. Immunol. by Sjöholm I. (1973)
10.1111/j.1432-1033.1972.tb02009.x
10.1111/j.1432-1033.1974.tb03717.x
{'key': 'e_1_2_2_12_2', 'first-page': '466', 'volume': '75', 'author': 'Forsgren A.', 'year': '1969', 'journal-title': 'Acta Pathol. Microbiol. Scand.'} / Acta Pathol. Microbiol. Scand. by Forsgren A. (1969)
10.1016/S0076-6879(67)11090-2
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10.1021/bi00840a007
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10.1021/bi00847a012
{'key': 'e_1_2_2_19_2', 'first-page': '173', 'volume-title': 'Conformation of Biopolymers', 'author': 'Timasheff S. N.', 'year': '1967'} / Conformation of Biopolymers by Timasheff S. N. (1967)
10.1016/B978-0-12-598650-2.50009-0
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10.1021/bi00863a036

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 10:54 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 5:56 p.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 8:59 a.m.)
Issued	50 years, 7 months ago (Feb. 1, 1975)
Published	50 years, 7 months ago (Feb. 1, 1975)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	50 years, 7 months ago (Feb. 1, 1975)

Funders 0

None

BibTeX

@article{SJ_HOLM_1975, title={Protein A from Staphylococcus aureus: Spectropolarimetric and Spectrophotometric Studies}, volume={51}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1975.tb03906.x}, DOI={10.1111/j.1432-1033.1975.tb03906.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SJÖHOLM, Ingvar}, year={1975}, month=feb, pages={55–61} }

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