DOI: 10.1111/j.1432-1033.1975.tb02391.x. The Amino‐Acid Sequence of Isoinhibitor K from Snails (<i>Helix pomatia</i>)

The Amino‐Acid Sequence of Isoinhibitor K from Snails (Helix pomatia)

10.1111/j.1432-1033.1975.tb02391.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Performic‐acid‐oxidized isoinhibitor K of snails (Helix pomatia) was subjected to argininedirected tryptic proteolysis. Six peptide fragments including one overlap peptide from limited cleavage of the Arg‐3‐Pro‐4 bond were purified to homogeneity. Four arginine peptides and the C‐terminal peptide were sequenced by automatic Edman degradation using a special peptide program. The phenylthiohydantoins were all identified by chemical ionization mass spectrometry, except four cysteic acid residues that were identified on an amino acid analyzer after acid hydrolysis. Quantitative evaluation of the phenylthiohydantoins by chemical ionization mass spectrometry using total molecular‐ion beam integration greatly facilitated sequencing. The mass spectrum of the dipeptide <Glu‐Gly revealed that the N‐terminus was blocked by pyroglutamic acid.The complete amino acid sequence of isoinhibitor K was determined. An almost 50% homology between the sequences of the snail inhibitor and the bovine trypsin‐kallikrein® inhibitor (Kunitz) became obvious. A comparison of all homologous sequences of this particular class of proteins known to date is presented.

Bibliography

TSCHESCHE, H., & DIETL, T. (1975). The AminoâAcid Sequence of Isoinhibitor K from Snails (Helix pomatia). European Journal of Biochemistry, 58(2), 439â451. Portico.

Authors 2

Harald TSCHESCHE (first)
Thomas DIETL (additional)

References 40 Referenced 30

10.1515/bchm2.1972.353.1.483 / HoppeSeyler's Z. Physiol. Chem. by Tschesche H. (1972)
10.1111/j.1432-1033.1972.tb02127.x
{'key': 'e_1_2_3_4_2', 'first-page': '437', 'volume': '20', 'author': 'Tschesche H.', 'year': '1973', 'journal-title': 'Protides Biol. Fluids, Proc. Colloq. Bruges'} / Protides Biol. Fluids, Proc. Colloq. Bruges by Tschesche H. (1973)
{'key': 'e_1_2_3_5_2', 'first-page': '1189', 'volume': '353', 'author': 'Tschesche H.', 'year': '1972', 'journal-title': "Hoppe-Seyler's Z. Physiol. Chem."} / Hoppe-Seyler's Z. Physiol. Chem. by Tschesche H. (1972)
10.1007/978-3-642-87966-1_30 / Proteinase Inhibitors, 2nd Int. Res. Conf. ‐ Bayer Symp. V by Dietl T. (1974)
10.1111/j.1432-1033.1975.tb02392.x
Tschesche H.(1976)Methods Enzymol. in press.
10.1085/jgp.19.6.991
10.1016/S0021-9258(18)65721-7
10.1042/bj1090312
{'key': 'e_1_2_3_12_2', 'first-page': '590', 'volume': '25', 'author': 'Yankelow J.', 'year': '1966', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Yankelow J. (1966)
10.1021/bi00841a033
10.1016/S0021-9258(18)65722-9
10.1021/ac60139a006
10.1016/S0021-9673(01)91862-X
10.1111/j.1432-1033.1967.tb00047.x
10.1002/oms.1210040149
10.1111/j.1432-1033.1970.tb01071.x
{'key': 'e_1_2_3_20_2', 'first-page': '135', 'volume-title': 'Proc. Int. Res. Conf. Proteinase Inhibitors', 'author': 'Tschesche H.', 'year': '1971'} / Proc. Int. Res. Conf. Proteinase Inhibitors by Tschesche H. (1971)
10.1021/bi00802a013
Reference deleted.
10.1007/978-3-642-87966-1_27 / Proteinase Inhibitors, 2nd Int. Res. Conf. ‐ Bayer Symp. V by Tschesche H. (1974)
Reference deleted.
10.1111/j.1432-1033.1972.tb01735.x
10.1016/S0021-9258(18)65784-9
{'key': 'e_1_2_3_27_2', 'first-page': '593', 'volume': '24', 'author': 'Kassell B.', 'year': '1965', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Kassell B. (1965)
10.1515/bchm2.1969.350.2.1247
10.1016/0006-291X(64)90433-4
10.1016/0006-291X(65)90601-7
{'key': 'e_1_2_3_31_2', 'first-page': '200', 'volume': '92', 'author': 'Chauvet J.', 'year': '1964', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta by Chauvet J. (1964)
10.1135/cccc19681363
10.1515/znb-1965-0511
{'key': 'e_1_2_3_34_2', 'first-page': '105', 'volume-title': 'Proc. Int. Res. Conf. Proteinase Inhibitors', 'author': 'Cechová D.', 'year': '1971'} / Proc. Int. Res. Conf. Proteinase Inhibitors by Cechová D. (1971)
10.1007/978-3-642-87966-1_66 / Proteinase Inhibitors, 2nd Int. Res. Conf.‐Bayer Symp. V by Laskowski M. (1974)
10.1007/978-3-642-87966-1_31 / Proteinase Inhibitors, 2nd Int. Res. Conf. ‐ Bayer Symp. V by Takahashi H. (1974)
10.1038/243088a0
{'key': 'e_1_2_3_38_2', 'first-page': '56', 'volume-title': 'Proc. Int. Res. Conf. Proteinase Inhibitors', 'author': 'Huber R.', 'year': '1971'} / Proc. Int. Res. Conf. Proteinase Inhibitors by Huber R. (1971)
10.1111/j.1432-1033.1968.tb00180.x
10.1007/978-3-642-87966-1_55 / Proteinase Inhibitors, 2nd Int. Res. Conf. Bayer Symp. V by Huber R. (1974)
10.1016/S0021-9258(19)41756-0

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:06 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 9:59 p.m.)
Indexed	1 year, 3 months ago (May 9, 2024, 1:54 a.m.)
Issued	49 years, 10 months ago (Oct. 1, 1975)
Published	49 years, 10 months ago (Oct. 1, 1975)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	49 years, 10 months ago (Oct. 1, 1975)

Funders 0

None

BibTeX

@article{TSCHESCHE_1975, title={The Amino‐Acid Sequence of Isoinhibitor K from Snails (Helix pomatia): A Sequence Determination by Automated Edman Degradation and Mass‐Spectral Identification of the Phenylthiohydantoins}, volume={58}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1975.tb02391.x}, DOI={10.1111/j.1432-1033.1975.tb02391.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={TSCHESCHE, Harald and DIETL, Thomas}, year={1975}, month=oct, pages={439–451} }

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