DOI: 10.1111/j.1432-1033.1975.tb02362.x. Control of Fatty‐Acid‐Synthetase Biosynthesis in <i>Saccharomyces cerevisiae</i>

Control of Fatty‐Acid‐Synthetase Biosynthesis in Saccharomyces cerevisiae

10.1111/j.1432-1033.1975.tb02362.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

143 out of 308 fasl mutants (47%) and 139 out of the 443 fas2‐mutants (32%) genetically studied in this laboratory fail to complement with any other fas‐mutant (deficient in fatty acid synthetase) of the same gene locus. From these noncomplementing fas‐mutants no mutant fatty acid synthetase can be isolated using the wild‐type enzyme purification procedure. Furthermore the noncomplementing fas‐mutants generally contain no material immunologically crossreacting with a specific fatty acid synthetase antiserum. However, subunits obtained after dissociation of the complex with sodium dodecylsulfate still cross react with this antiserum. Therefore, it is concluded that noncomplementing fas‐mutants contain no fatty acid synthetase component proteins, though one of the two fas‐loci is mutationally unaffected. This conclusion was further confirmed by 14C‐labeled amino acid incorporation studies which indicated that in noncomplementing fas‐mutants, other than in wild type and complementing fas‐mutant cells, no label was incorporated into fatty acid synthetase subunits or precursor proteins. At nonpermissive temperature, the same biochemical and immunological characteristics were observed with temperature‐sensitive non‐complementing fas‐mutants.These results suggest that noncomplementing fas‐mutants either represent regulatory mutants unable to induce the mutationally unaffected other fas‐gene locus or that they are association‐defective mutants. In both cases the resulting individual subunits of the complex may be rapidly degraded by intracellular proteases.

Bibliography

DIETLEIN, G., & SCHWEIZER, E. (1975). Control of FattyâAcidâSynthetase Biosynthesis in Saccharomyces cerevisiae. European Journal of Biochemistry, 58(1), 177â184. Portico.

Authors 2

Gerhard DIETLEIN (first)
Eckhart SCHWEIZER (additional)

References 14 Referenced 17

10.1111/j.1432-1033.1973.tb03133.x
{'key': 'e_1_2_1_3_2', 'first-page': '219', 'volume-title': 'Comparative Biochemistry and Physiology of Transport', 'author': 'Schweizer E.', 'year': '1974'} / Comparative Biochemistry and Physiology of Transport by Schweizer E. (1974)
10.1111/j.1432-1033.1972.tb19711.x
10.1007/BF00272089 / Mol. Gen. Genet. by Burkl G. (1972)
10.1515/bchm2.1971.352.1.377
10.1007/BF00268627
10.1073/pnas.67.2.660
10.1016/S0022-2836(64)80156-X
10.1016/0006-291X(70)90034-3
10.1146/annurev.bi.43.070174.004155
10.1016/S0021-9258(19)45513-0 / J. Biol. Chem. by Lin S. (1972)
10.1016/0022-5193(72)90026-4
10.1002/9780470122822.ch3
{'key': 'e_1_2_1_15_2', 'first-page': '236', 'volume-title': 'Enzyme Synthesis and Degradation in Mammalian Systems', 'author': 'Rechcigl M.', 'year': '1971'} / Enzyme Synthesis and Degradation in Mammalian Systems by Rechcigl M. (1971)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:03 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:10 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 2:41 p.m.)
Issued	49 years, 10 months ago (Oct. 1, 1975)
Published	49 years, 10 months ago (Oct. 1, 1975)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	49 years, 10 months ago (Oct. 1, 1975)

Funders 0

None

BibTeX

@article{DIETLEIN_1975, title={Control of Fatty‐Acid‐Synthetase Biosynthesis in Saccharomyces cerevisiae}, volume={58}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1975.tb02362.x}, DOI={10.1111/j.1432-1033.1975.tb02362.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={DIETLEIN, Gerhard and SCHWEIZER, Eckhart}, year={1975}, month=oct, pages={177–184} }

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