DOI: 10.1111/j.1432-1033.1974.tb03733.x. Studies on Polysome‐Membrane Interactions in Mouse Myeloma Cells

Studies on Polysome‐Membrane Interactions in Mouse Myeloma Cells

10.1111/j.1432-1033.1974.tb03733.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The nature of the interaction of membrane‐bound polysomes with the endoplasmic reticulum has been studied in MOPC 21 mouse plasmocytoma tissue‐culture cells which secrete an immunoglobulin G. Treatment of microsomes at high salt concentrations (500 mM KCl) results in the detachment of 40–45% of the bound ribosomes. This released (“loose”) fraction is predominantly monosomal in contrast to the remaining “tight” polysomes. It is shown that both ribosomal fractions contain immunoglobulin light‐chain mRNA which argues against a functional distinction between them in the cell.Polysomes have been dissociated to apparently undegraded ribosomal subunits at 0°C by incubation with puromycin in the presence of a magnesium ion buffer. Application of this procedure to membrane‐bound polysomes results in partial release of ribosomal subunits from the membrane. The ribosomal material which is not so released sediments predominantly at 60 S and this fraction can be detached by subsequent washing at 500 mM KCl. Thus, by use of the polysome‐dissociating procedure together with high salt concentrations, essentially total release of the ribosomal components can be obtained.The results indicate that the salt‐labile interactions are mediated via the large ribosomal subunits and are the only anchoring forces to the membrane at early stages of protein synthesis. The tight membrane‐bound polysomes result from the additional anchoring effect of the polypeptide chains as they grow through the membrane.

Bibliography

HARRISON, T. M., BROWNLEE, G. G., & MILSTEIN, C. (1974). Studies on PolysomeâMembrane Interactions in Mouse Myeloma Cells. European Journal of Biochemistry, 47(3), 613â620. Portico.

Authors 3

Timothy M. HARRISON (first)
George G. BROWNLEE (additional)
Cesar MILSTEIN (additional)

References 35 Referenced 56

10.1038/189558a0
10.1126/science.164.3879.584
10.1016/S0021-9258(18)94321-8
10.1016/S0021-9258(18)96815-8 / J. Biol. Chem. by Redman C. M. (1966)
10.1083/jcb.45.1.146
10.1073/pnas.58.6.2422
10.1073/pnas.59.2.445
10.1042/bj1220005
10.1016/S0022-2836(66)80019-0
10.1016/0022-2836(71)90048-9
10.1016/0022-2836(71)90049-0 / J. Mol. Biol. by Rosbash M. (1971)
10.1083/jcb.49.3.683
10.1083/jcb.56.1.206
10.1073/pnas.68.2.390
10.1016/0006-291X(69)90892-4
10.1002/9780470110195.ch10
10.1042/bj1260837
10.1016/0005-2787(72)90038-X
10.1038/227680a0
10.1016/0005-2787(69)90187-7
10.1038/newbio232008a0
10.1038/newbio239117a0
{'key': 'e_1_2_2_24_2', 'first-page': '361', 'volume-title': 'Stability Constants of Metal‐Ion Complexes', 'author': 'Martell A. E.', 'year': '1964'} / Stability Constants of Metal‐Ion Complexes by Martell A. E. (1964)
Harrison T. M.(1972)Ph. D. Thesis University of Cambridge.
10.1016/0022-2836(73)90407-5
10.1021/bi00831a013
10.1016/0022-2836(70)90042-2
10.1111/j.1432-1033.1974.tb03734.x
10.1016/0022-2836(73)90037-5
10.1016/0022-2836(72)90198-2
10.1073/pnas.66.2.425
10.1073/pnas.66.4.1183 / Proc. Natl. Acad. Sci. U.S.A. by Scherr C. J. (1970)
10.1016/0006-291X(66)90370-6
10.1016/S0022-2836(66)80124-9
{'key': 'e_1_2_2_36_2', 'first-page': '193', 'volume-title': 'Biomembranes', 'author': 'Blobel G.', 'year': '1972'} / Biomembranes by Blobel G. (1972)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 10:32 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 9:36 p.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 5:03 p.m.)
Issued	50 years, 11 months ago (Sept. 1, 1974)
Published	50 years, 11 months ago (Sept. 1, 1974)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	50 years, 11 months ago (Sept. 1, 1974)

Funders 0

None

BibTeX

@article{HARRISON_1974, title={Studies on Polysome‐Membrane Interactions in Mouse Myeloma Cells}, volume={47}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1974.tb03733.x}, DOI={10.1111/j.1432-1033.1974.tb03733.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={HARRISON, Timothy M. and BROWNLEE, George G. and MILSTEIN, Cesar}, year={1974}, month=sep, pages={613–620} }

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