DOI: 10.1111/j.1432-1033.1974.tb03648.x. Modification of Cysteine Residues with Sodium 2‐Bromoethanesulfonate

Modification of Cysteine Residues with Sodium 2‐Bromoethanesulfonate

10.1111/j.1432-1033.1974.tb03648.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A procedure for alkylation of cysteine residues with sodium 2‐bromoethanesulfonate is described. The reaction is performed under mild conditions and complete modification is obtained without side reactions. S‐Sulfoethylated proteins are comparable to performic acid oxidized or S‐sulfonated proteins with respect to solubility properties and behaviour in ion exchange chromatography. S‐Sulfoethylcysteine was found to be stable during acid hydrolysis. S‐Sulfoethylated peptides were suitable for automatic Edman degradation due to their polarity, since losses during extraction are reduced, especially after the coupling stage, as shown by degradation of modified peptides.

Bibliography

NIKETIC, V., THOMSEN, J., & KRISTIANSEN, K. (1974). Modification of Cysteine Residues with Sodium 2âBromoethanesulfonate. European Journal of Biochemistry, 46(3), 547â551. Portico.

Authors 3

Vesna NIKETIC (first)
Johannes THOMSEN (additional)
Karsten KRISTIANSEN (additional)

References 27 Referenced 15

{'key': 'e_1_2_2_2_2', 'first-page': '379', 'volume-title': 'The Proteins', 'author': 'Cecil R.', 'year': '1963'} / The Proteins by Cecil R. (1963)
10.1016/S0076-6879(67)11021-5
10.1111/j.1399-3011.1969.tb01653.x
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{'key': 'e_1_2_2_7_2', 'first-page': '1589', 'volume': '351', 'author': 'Braunitzer G.', 'year': '1970', 'journal-title': "Hoppe-Seyler's Z. Physiol. Chem."} / Hoppe-Seyler's Z. Physiol. Chem. by Braunitzer G. (1970)
{'key': 'e_1_2_2_8_2', 'first-page': '1730', 'volume': '352', 'author': 'Braunitzer G.', 'year': '1971', 'journal-title': "Hoppe-Seyler's Z. Physiol. Chem."} / Hoppe-Seyler's Z. Physiol. Chem. by Braunitzer G. (1971)
{'key': 'e_1_2_2_9_2', 'first-page': '558', 'volume': '2', 'author': 'Marvel C. S.', 'year': '1943', 'journal-title': 'Org. Synth. Coll.'} / Org. Synth. Coll. by Marvel C. S. (1943)
10.1016/0014-5793(72)80192-3
10.1016/0076-6879(63)06257-1
{'key': 'e_1_2_2_12_2', 'first-page': '16', 'volume-title': 'Methods for Determination of Amino‐Acid Composition of Milk Proteins', 'author': 'Brunfeldt K.', 'year': '1966'} / Methods for Determination of Amino‐Acid Composition of Milk Proteins by Brunfeldt K. (1966)
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10.1016/S0021-9258(18)81308-4
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10.1007/978-3-662-12834-3_8
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{'key': 'e_1_2_2_27_2', 'first-page': '150', 'volume-title': 'Kinetics and Mechanism', 'author': 'Frost A. A.', 'year': '1961'} / Kinetics and Mechanism by Frost A. A. (1961)
{'key': 'e_1_2_2_28_2', 'first-page': '374', 'volume-title': 'Physical Organic Chemistry', 'author': 'Wiberg K. B.', 'year': '1964'} / Physical Organic Chemistry by Wiberg K. B. (1964)

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 8:40 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 4:41 p.m.)
Indexed	2 days, 1 hour ago (Sept. 4, 2025, 9:48 a.m.)
Issued	51 years, 1 month ago (Aug. 1, 1974)
Published	51 years, 1 month ago (Aug. 1, 1974)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	51 years, 1 month ago (Aug. 1, 1974)

Funders 0

None

BibTeX

@article{NIKETIC_1974, title={Modification of Cysteine Residues with Sodium 2‐Bromoethanesulfonate: The Application of S‐Sulfoethylated Peptides in Automatic Edman Degradation}, volume={46}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1974.tb03648.x}, DOI={10.1111/j.1432-1033.1974.tb03648.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={NIKETIC, Vesna and THOMSEN, Johannes and KRISTIANSEN, Karsten}, year={1974}, month=aug, pages={547–551} }

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