DOI: 10.1111/j.1432-1033.1974.tb03607.x. Reassociation and Reactivation of Lactic Dehydrogenase from the Unfolded Subunits

Reassociation and Reactivation of Lactic Dehydrogenase from the Unfolded Subunits

10.1111/j.1432-1033.1974.tb03607.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Lactic dehydrogenase from pig heart (H4) shows pH‐dependent reversible deactivation, denaturation and dissociation at pH < 6.The initial and final states in the dissociation‐association process are found to be the homogeneous fully inactive monomer (Mr= 37500 ± 1000) on one hand, and a heterogeneous mixture of denatured subunits, native tetramers and inactive higher aggregates on the other hand. The yield of tetramers and the extent to which renaturation occurs correspond to each other.Using optimum conditions of reactivation [pH 7.9, I∼ 0.2, 20 °C, in the presence of 1 mM dithiothreitol and 10 mM EDTA, enzyme concentration below 0.05 mg/ml], the correlation of reactivation and reassociation may be analyzed. The second‐order kinetics of the reactivation process prove reassociation to be the rate‐limiting step in the process of reactivation. From this one may conclude that in the case of the heart isoenzyme of lactic dehydrogenase the subunit does not represent the enzymatically active unit.The coenzyme (NAD+) increases the rate of reactivation but not the overall recovery. This suggests the coenzyme is involved in a nucleation step in the process of refolding and/or reassociation.

Bibliography

JAENICKE, R. (1974). Reassociation and Reactivation of Lactic Dehydrogenase from the Unfolded Subunits. European Journal of Biochemistry, 46(1), 149â155. Portico.

Authors 1

Rainer JAENICKE (first)

References 25 Referenced 63

10.1146/annurev.bi.42.070173.001031
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Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 8:34 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 3:24 p.m.)
Indexed	2 days, 4 hours ago (Sept. 4, 2025, 10:17 a.m.)
Issued	51 years, 2 months ago (July 1, 1974)
Published	51 years, 2 months ago (July 1, 1974)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	51 years, 2 months ago (July 1, 1974)

Funders 0

None

BibTeX

@article{JAENICKE_1974, title={Reassociation and Reactivation of Lactic Dehydrogenase from the Unfolded Subunits}, volume={46}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1974.tb03607.x}, DOI={10.1111/j.1432-1033.1974.tb03607.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={JAENICKE, Rainer}, year={1974}, month=jul, pages={149–155} }

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