DOI: 10.1111/j.1432-1033.1973.tb03082.x. Comparative Study of Photophosphorylation Coupling Factor · Ligand Complex by Circular Dichroism and Chemical Isolation

Comparative Study of Photophosphorylation Coupling Factor · Ligand Complex by Circular Dichroism and Chemical Isolation

10.1111/j.1432-1033.1973.tb03082.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

1. The interaction between the protein coupling of photophosphorylation and its substrate ADP is studied comparatively by two methods: circular dichroism and chemical isolation of the ligand · enzyme complex.2. Experiments with 14C‐labelled ADP indicate that the amount of bound nucleotide is proportional to a variation of the circular dichroism spectrum around 260 nm. When coupling factor is saturated with ADP, two moles of ADP are bound per mole of coupling factor.3. The fixation of ADP on coupling factor is inhibited by the presence, in the incubation medium, of phosphate (Pi), pyrophosphate (PPi) or tripolyphosphate ions. The incubation of coupling factor with [32P]PPi shows that PPi inhibition is due to its fixation on the coupling factor. When coupling factor is saturated, two moles of PPi are bound per mole of coupling factor.

Bibliography

Girault, G., Galmiche, J., MichelâVillaz, M., & Thiery, J. (1973). Comparative Study of Photophosphorylation Coupling Factor Â· Ligand Complex by Circular Dichroism and Chemical Isolation. European Journal of Biochemistry, 38(3), 473â478. Portico.

Authors 4

Guy Girault (first)
Jean‐Michel Galmiche (additional)
Michel Michel‐Villaz (additional)
Jean Thiery (additional)

References 13 Referenced 50

{'key': 'e_1_2_1_2_2', 'first-page': '543', 'volume-title': 'Actes du Colloque internation n° 119 du Centre National de la Recherche Scientifique', 'author': 'Avron M.', 'year': '1963'} / Actes du Colloque internation n° 119 du Centre National de la Recherche Scientifique by Avron M. (1963)
10.1016/S0021-9258(18)97376-X
10.1073/pnas.58.3.1261
10.1021/bi00821a023
10.1016/S0021-9258(18)99333-6
10.1073/pnas.68.2.464
10.1073/pnas.68.11.2720
10.1016/0014-5793(72)80400-9
10.1016/S0021-9258(18)91846-6
{'key': 'e_1_2_1_11_2', 'first-page': '1145', 'volume-title': 'Proceeding of the 2nd International Congress on Photosynthesis Research', 'author': 'Girault G.', 'year': '1971'} / Proceeding of the 2nd International Congress on Photosynthesis Research by Girault G. (1971)
10.1016/S0021-9258(18)91847-8 / J. Biol. Chem. by Livne A. (1966)
10.1111/j.1749-6632.1949.tb27297.x
10.1016/0003-2697(72)90084-X

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 6:55 a.m.)
Deposited	1 year, 11 months ago (Oct. 1, 2023, 4:22 a.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 6:14 a.m.)
Issued	51 years, 11 months ago (Oct. 1, 1973)
Published	51 years, 11 months ago (Oct. 1, 1973)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	51 years, 11 months ago (Oct. 1, 1973)

Funders 0

None

BibTeX

@article{Girault_1973, title={Comparative Study of Photophosphorylation Coupling Factor · Ligand Complex by Circular Dichroism and Chemical Isolation}, volume={38}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1973.tb03082.x}, DOI={10.1111/j.1432-1033.1973.tb03082.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Girault, Guy and Galmiche, Jean‐Michel and Michel‐Villaz, Michel and Thiery, Jean}, year={1973}, month=oct, pages={473–478} }

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