DOI: 10.1111/j.1432-1033.1973.tb02994.x. On the Reconstitution of Photophosphorylation in Chloroplast Membranes

On the Reconstitution of Photophosphorylation in Chloroplast Membranes

10.1111/j.1432-1033.1973.tb02994.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A high degree of reconstitution of photophosphorylation was achieved using resolved chloroplast particles obtained by incubation with 1 mM EDTA at relatively high chlorophyll concentrations (about 400 μg/ml). The resolved particles lost about 95% and 75% of their phosphorylative and proton transport activity respectively. Upon reconstitution with either crude or purified chloroplast coupling factor preparations, almost full activity was recovered. Control of electron transport by phosphorylating agents was also observed in these reconstituted preparations.Highly reconstituted partieles were also obtained by incubation of chloroplasts, at low chlorophyll concentrations, with EDTA up to 10 mM. Above this concentration of EDTA, poor uncoupling was observed.Gel electrophoresis of supernatant fractions obtained from incubations with EDTA under conditions which produced resolved particles with a poor degree of reconstitution revealed a protein fraction not appearing in supernatants from highly reconstituted particles. The degree of reconstitution was inversely correlated with the appearance of this protein band.

Bibliography

Shoshan, V., & Shavit, N. (1973). On the Reconstitution of Photophosphorylation in Chloroplast Membranes. European Journal of Biochemistry, 37(2), 355â360. Portico.

Authors 2

Varda Shoshan (first)
Noun Shavit (additional)

References 23 Referenced 29

10.1016/0006-3002(63)90567-5
10.1016/S0021-9258(18)97376-X
10.1021/bi00821a024
10.1016/S0021-9258(18)99333-6
10.1016/S0021-9258(19)44721-2
10.1016/S0076-6879(71)23099-8
10.1016/0003-9861(72)90223-8
10.1016/0006-3002(60)91350-0
10.1016/0003-2697(63)90149-0
10.1016/0003-9861(64)90276-0
10.1016/S0021-9258(18)91846-6
10.1021/bi00821a023
10.1016/S0021-9258(19)52451-6
10.1104/pp.24.1.1
10.1111/j.1749-6632.1964.tb14207.x
10.1111/j.1749-6632.1964.tb14213.x
10.1073/pnas.67.2.477
{'key': 'e_1_2_2_19_2', 'first-page': '202', 'volume': '19', 'author': 'McCarty R. E.', 'year': '1966', 'journal-title': 'Brookhaven Symp. Biol.'} / Brookhaven Symp. Biol. by McCarty R. E. (1966)
10.1016/S0021-9258(19)45171-5
10.1016/S0021-9258(19)44575-4
10.1021/bi00769a001
{'key': 'e_1_2_2_23_2', 'first-page': '1221', 'volume-title': 'Proc. Int. Congr. Photosynthesis Res.', 'author': 'Shavit N.', 'year': '1972'} / Proc. Int. Congr. Photosynthesis Res. by Shavit N. (1972)
10.1073/pnas.68.10.2522

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 6:33 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 5:07 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 5:59 p.m.)
Issued	52 years ago (Aug. 1, 1973)
Published	52 years ago (Aug. 1, 1973)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	52 years ago (Aug. 1, 1973)

Funders 0

None

BibTeX

@article{Shoshan_1973, title={On the Reconstitution of Photophosphorylation in Chloroplast Membranes}, volume={37}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1973.tb02994.x}, DOI={10.1111/j.1432-1033.1973.tb02994.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Shoshan, Varda and Shavit, Noun}, year={1973}, month=aug, pages={355–360} }

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