DOI: 10.1111/j.1432-1033.1973.tb02877.x. The Translation <i>in vitro</i> of mRNA for Immunoglobulin Heavy Chains

The Translation in vitro of mRNA for Immunoglobulin Heavy Chains

10.1111/j.1432-1033.1973.tb02877.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The addition of mRNA preparations to a number of heterologous cell‐free systems has been shown to result in the synthesis of immunoglobulin light chains. This paper reports the results of experiments in which an mRNA fraction prepared from MOPC 21 mouse myeloma cells has been used to stimulate the synthesis of immunoglobulin heavy chains in rabbit reticulocyte lysates. The synthesis of heavy chains in a homologous cell‐free system containing either microsomes or membrane‐derived polysomes is also described. The synthetic product has been characterised by finger‐printing in each case. The results reveal the presence of a unique methionine‐containing peptide in digests of the product of the rabbit reticulocyte cell‐free system. This peptide is not observed in corresponding digests of heavy chains synthesized in vivo or in the homologous cell‐free system in vitro.

Bibliography

Cowan, N. J., & Milstein, C. (1973). The Translation in vitro of mRNA for Immunoglobulin Heavy Chains. European Journal of Biochemistry, 36(1), 1â7. Portico.

Authors 2

Nicholas Justin Cowan (first)
Cesar Milstein (additional)

References 25 Referenced 28

10.1016/0006-291X(69)90720-7
10.1016/0006-291X(72)90725-5
10.1016/S0021-9258(19)45901-2
10.1016/0006-291X(72)90948-5
10.1038/newbio236007a0
10.1073/pnas.69.8.2095
10.1101/SQB.1969.034.01.091
10.1038/newbio230172a0
10.1038/newbio239117a0
10.1016/0014-5793(72)80352-1
10.1016/0022-2836(71)90080-5
10.1042/bj1260837
10.1073/pnas.69.2.417
10.1038/227680a0
10.1016/0042-6822(59)90043-1
10.1038/239143a0
10.1073/pnas.69.7.1967
Reference deleted.
10.1016/0006-291X(72)90478-0
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10.1042/bj1190765
10.1016/0014-4827(70)90489-1
10.1016/0006-291X(69)90536-1
10.1016/S0021-9258(18)97160-7 / J. Biol. Chem. by McGuire E. J. (1965)
10.1042/bj0890341

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 10:21 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 3:01 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:40 p.m.)
Issued	52 years, 2 months ago (July 1, 1973)
Published	52 years, 2 months ago (July 1, 1973)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	52 years, 2 months ago (July 1, 1973)

Funders 0

None

BibTeX

@article{Cowan_1973, title={The Translation in vitro of mRNA for Immunoglobulin Heavy Chains}, volume={36}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1973.tb02877.x}, DOI={10.1111/j.1432-1033.1973.tb02877.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Cowan, Nicholas Justin and Milstein, Cesar}, year={1973}, month=jul, pages={1–7} }

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